Immunity to SARS-CoV-2 up to 15 months after infection

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1. Version published to 10.1016/j.isci.2022.103743

   Feb 1, 2022
2. 

   ScreenIT

   Oct 13, 2021

   SciScore for 10.1101/2021.10.08.463699: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Detection of antibodies specific to SARS-CoV-2: Levels of anti-S and anti-RBD IgM, IgA and IgG antibodies were determined by ELISA as previously described6.	anti-S suggested: None anti-RBD IgM , IgA and IgG suggested: None
   Quantification and Statistical analysis: Mann-Whitney U test was used for comparisons between groups in anti-SARS-CoV-2 antibody levels, neutralization titers and numbers of specific memory B and T cells.	anti-SARS-CoV-2 suggested: None
   Experimental Models: Cell Lines
   Sentenc…

   SciScore for 10.1101/2021.10.08.463699: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Detection of antibodies specific to SARS-CoV-2: Levels of anti-S and anti-RBD IgM, IgA and IgG antibodies were determined by ELISA as previously described6.	anti-S suggested: None anti-RBD IgM , IgA and IgG suggested: None
   Quantification and Statistical analysis: Mann-Whitney U test was used for comparisons between groups in anti-SARS-CoV-2 antibody levels, neutralization titers and numbers of specific memory B and T cells.	anti-SARS-CoV-2 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Production of SARS-CoV-2 RBD and S proteins: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S) protein from Wuhan-Hu-1 strain and RBD from variants (Alpha, Beta, Gamma, and Delta) were expressed baculovirus-free in High Five insect cells52 and purified on HisTrap excel column (Cytiva) followed by preparative size exclusion chromatography on 16/600 Superdex 200 pg column (Cytiva)53,54.	Expi293 suggested: RRID:CVCL_D615)
   Software and Algorithms
   Sentences	Resources
   High-binding Corning Half area plates (Corning #3690) were coated overnight at 4°C with purified S or RBD protein derived from wild-type virus (1.7 μg/ml for IgM and IgG; 2.0 μg/ml for IgA) in PBS.	IgG suggested: (DSHB Cat# LEP100 IgG, RRID:AB_528124)
   Results of ELISpot and FluoroSpot assays were evaluated using an IRIS-reader and analyzed by the IRIS software version 1.1.9 (Mabtech AB).	IRIS suggested: None
   All analyses and data plotting were performed using GraphPad or R version 3.6.1.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.10.08.463699v1 on bioRxiv

   Oct 11, 2021