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  1. Cell walls are dynamically O-acetylated in the green seaweed, Ulva compressa

    1. John H. Bothwell
    2. Alexander J. Goodridge
    3. Marie Rapin
    4. Patrick J. Brennan
    5. Alexandra Traslaviña López
    6. Akanksha Agrawal
    7. Stephen C. Fry
    8. Georgia Campbell
    9. Jonas Blomme
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    An interesting paper identifying stress-induced modification (O-acetylation) in the cell wall of the green seaweed, Ulva compressa. It contains a protocol for chlorophyte cell wall fractionation.

  2. Signal Peptide Efficiency: from High-throughput Data to Prediction and Explanation

    1. Stefano Grasso
    2. Valentina Dabene
    3. Margriet M.W.B. Hendriks
    4. Priscilla Zwartjens
    5. René Pellaux
    6. Martin Held
    7. Sven Panke
    8. Jan Maarten van Dijl
    9. Andreas Meyer
    10. Tjeerd van Rij
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    Whoa nice, a systematic analyses of thousands of signal peptide sequences and effects on properties affecting secretion

  3. ERK pathway activation inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement

    1. Larissa L Dougherty
    2. Soumita Dutta
    3. Prachee Avasthi
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    Again I get to highlight one of our new preprints! This one tackles the intricate interplay between cell division and ciliogenesis. Using genetics, biochemistry, four different imaging modalities, and two organisms, we identified cytoskeletal, motor, and trafficking defects causing impaired cilium formation when activating a major cell proliferation and cancer pathway.

  4. Blind spots on western blots: a meta-research study highlighting opportunities to improve figures and methods reporting

    1. Cristina Kroon
    2. Larissa Breuer
    3. Lydia Jones
    4. Jeehye An
    5. Ayça Akan
    6. Elkhansa Ahmed Mohamed Ali
    7. Felix Busch
    8. Marinus Fislage
    9. Biswajit Ghosh
    10. Max Hellrigel-Holderbaum
    11. Vartan Kazezian
    12. Alina Koppold
    13. Cesar Alberto Moreira Restrepo
    14. Nico Riedel
    15. Lea Scherschinski
    16. Fernando Raúl Urrutia Gonzalez
    17. Tracey Weissgerber
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    There may not be a technique as over-interpreted relative to the information that it can provide as a western blot but this is made worse by ignoring best practices and my favorite, using a single protein as an unvalidated loading control. This analysis of westerns from the literature highlights some better display and reporting practices

  5. Liquid-like assembly of VASP drives actin polymerization and bundling

    1. Kristin Graham
    2. Aravind Chandrasekaran
    3. Liping Wang
    4. Aly Ladak
    5. Eileen M. Lafer
    6. Padmini Rangamani
    7. Jeanne C. Stachowiak
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    I have no idea whether this actually happens in a cell but what an interesting model! I think I audibly gasped at figure 3A.

  6. Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast

    1. Kerstin Hinterndorfer
    2. Marine. H. Laporte
    3. Felix Mikus
    4. Lucas Tafur Petrozzi
    5. Clélia Bourgoint
    6. Manoel Prouteau
    7. Gautam Dey
    8. Robbie Loewith
    9. Paul Guichard
    10. Virginie Hamel
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    Spectacular expansion microscopy in yeast! I went straight for the cell wall digestion protocol so we can try it in a number of different algae

  7. Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion

    1. Ingrid Billault-Chaumartin
    2. Olivia Muriel
    3. Laetitia Michon
    4. Sophie G Martin
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    This is so cool! The intrinsically disordered region in a pombe formin is needed to generate the foci needed for actin mediated cell fusion. Both replacement and rescue generate the expected behaviors associated with condensation state. Lots of interesting papers recently on condensates mediating behaviors of actin regulators and polymerization!

  8. Protrusion growth driven by myosin-generated force

    1. Gillian N. Fitz
    2. Meredith L. Weck
    3. Caroline Bodnya
    4. Olivia L. Perkins
    5. Matthew J. Tyska
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    Interesting study showing that membrane-anchored myosin can generate the force necessary for membrane protrusion beyond actin polymerization based mechanisms

  9. Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL

    1. Thomas L. Steinacker
    2. Siu-Shing Wong
    3. Zsofia A. Novak
    4. Saroj Saurya
    5. Lisa Gartenmann
    6. Eline J.H. van Houtum
    7. Judith R. Sayers
    8. B. Christoffer Lagerholm
    9. Jordan W. Raff
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    Limiting component models in organelle size control are difficult to pin down as the limiting components themselves are hard to identify. This study identifies an alternate kinase-dependent model for centriole size control.

  10. Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis

    1. Brae M Bigge
    2. Prachee Avasthi
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    This week, I get to highlight our own preprint proposing a putative mechanism of lithium-mediated cilium extension through stimulating endocytosis to supply membrane to the cilium from the plasma membrane. We proposed this rapid route for ciliary material in our last preprint

  11. Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Fornicata

    1. Rui Santos
    2. Ásgeir Ástvaldsson
    3. Shweta V. Pipaliya
    4. Jon Paulin Zumthor
    5. Joel B. Dacks
    6. Staffan Svärd
    7. Adrian B. Hehl
    8. Carmen Faso
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    Incredibly interesting study of endocytic adaptation in an obligate parasite in which a structural analog of clathrin light chain is predicted/identified.

  12. Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection

    1. Amadeus Xu
    2. Angika Basant
    3. Sibylle Schleich
    4. Timothy P Newsome
    5. Michael Way
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    Do yourself a favor and check out this exquisite characterization of vaccinia viron transport towards microtubule plus ends. In-cell deletion and microscopy, in vitro reconstitution, quantification of motor complexes associated with virons and viron speed … really beautiful work!

  13. Conserved degronome features governing quality control-associated proteolysis

    1. Bayan Mashahreh
    2. Shir Armony
    3. Kristoffer Enøe Johansson
    4. Alon Chapelbaum
    5. Nir Friedman
    6. Richard G. Gardner
    7. Rasmus Hartmann-Petersen
    8. Kresten Lindorff-Larsen
    9. Tommer Ravid
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    How interesting! A peptide screen to identify degrons with E3 ubiquitin ligase specificity in yeast followed by extraction of conserved properties identifies transmembrane domain containing peptides as likely degrons.

  14. Integrating bulk and single cell RNA-seq refines transcriptomic profiles of specific C. elegans neurons

    1. Alec Barrett
    2. Erdem Varol
    3. Alexis Weinreb
    4. Seth R. Taylor
    5. Rebecca M. McWhirter
    6. Cyril Cros
    7. Manasa Basaravaju
    8. Abigail Poff
    9. John A. Tipps
    10. Maryam Majeed
    11. Berta Vidal
    12. Chen Wang
    13. Eviatar Yemini
    14. Emily A. Bayer
    15. HaoSheng Sun
    16. Oliver Hobert
    17. David M. Miller
    18. Marc Hammarlund
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    Recognizing the utility of not using exclusively cutting edge methods for everything, this important study combines bulk RNAseq (high sensitivity due to capturing more low expression and noncoding RNAs) and single cell RNAseq (high specificity) for a more comprehensive transcriptomic picture.

  15. Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii

    1. Raheel Ahmad
    2. Albert J Bae
    3. Yu-Jung Su
    4. Samira Goli Pozveh
    5. Eberhard Bodenschatz
    6. Alain Pumir
    7. Azam Gholami
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    This is wild! Chlamydomonas flagella were isolated, demembranated, attached to polystyrene beads and reactivated to reconstitute flagellar movement. This yields some interesting insights through experimentation and simulation: Increased calcium concentration affects flagellar waveform and trajectory of bead movement and surprisingly, larger beads have increased rather than decreased velocity.

  16. ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants

    1. Jan Martinek
    2. Petra Cifrová
    3. Stanislav Vosolsobě
    4. Jana Krtková
    5. Lenka Sikorová
    6. Kateřina Malínská
    7. Zdeňka Mauerová
    8. Ian Leaves
    9. Imogen Sparkes
    10. Kateřina Schwarzerová
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    Interesting role for ARP2/3 complex in peroxisome autophagy in plants!