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  1. Universal length fluctuations of actin structures found in cells

    This article has 5 authors:
    1. Aldric Rosario
    2. Shane G. McInally
    3. Predrag R. Jelenkovic
    4. Bruce L. Goode
    5. Jane Kondev
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is a theoretical analysis that gives compelling evidence that length control of bundles of actin filaments undergoing assembly and disassembly emerges even in the absence of a length control mechanism at the individual filament level. Furthermore, the length distribution should exhibit a variance that grows quadratically with the average bundle length. The experimental data are compatible with these fundamental theoretical findings, but further investigations are necessary to make the work conclusive concerning the validity of the inferences for filamentous actin structures in cells.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 3 listsLatest version Latest activity
  2. A Novel Imaging Method (FIM-ID) Reveals that Myofibrillogenesis Plays a Major Role in the Mechanically Induced Growth of Skeletal Muscle

    This article has 11 authors:
    1. Kent W. Jorgenson
    2. Jamie E. Hibbert
    3. Ramy K. A. Sayed
    4. Anthony N. Lange
    5. Joshua S. Godwin
    6. Paulo H. C. Mesquita
    7. Bradley A. Ruple
    8. Mason C. McIntosh
    9. Andreas N. Kavazis
    10. Michael D. Roberts
    11. Troy A. Hornberger
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The work by Hornberger and team presents a novel workflow for the visualisation of myofibrils with high resolution and contrast that will be extremely valuable for the scientific community. The novel methods include solid validation of both sample preparation and analysis, and have been used to make the fundamental discovery of myofibrillogenesis as the mechanism of mechanical loading-induced growth. However, whether this mechanism is present in other settings of muscle growth (i.e non-loading), other striated tissue (e.g myocardium), or is sex-dependent requires future experiments.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. Optogenetic control of a GEF of RhoA uncovers a signaling switch from retraction to protrusion

    This article has 4 authors:
    1. Jean De Seze
    2. Maud Bongaerts
    3. Benoit Boulevard
    4. Mathieu Coppey
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study combines experiments with optogenetic actuation and theory to understand how signalling proteins control the switch between cell protrusion and retraction, two fundamental processes in single-cell migration. The authors examine the role of a guanine exchange factor (GEF) on the downstream effectors RhoA and Cdc42, which trigger retraction and protrusion, respectively. The experimental and theoretical evidence provides a convincing explanation for why RhoA is involved in both protrusion and retraction.

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    This article has 6 evaluationsAppears in 2 listsLatest version Latest activity
  4. Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

    This article has 5 authors:
    1. Rajdeep Banerjee
    2. Thomas J. Meyer
    3. Margaret C. Cam
    4. Sukhbir Kaur
    5. David D. Roberts
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable finding on the cell composition in mouse spleen depleted for the CD47 receptor and its signaling ligand Thrombospondin in hematopoietic differentiation. The supporting evidence is convincing with analytical improvements on the individual contributions of the signaling components and with functional studies. This work has implications for the role of CD47/Thsp in extramedullary erythropoiesis in mouse spleen and will be of interest to researchers in cell signaling, transfusion medicine, and cell therapy.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  5. Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids

    This article has 10 authors:
    1. Xiuling Cao
    2. Xiang Wu
    3. Lei Zhao
    4. Ju Zheng
    5. Xuejiao Jin
    6. Xinxin Hao
    7. Joris Winderickx
    8. Shenkui Liu
    9. Lihua Chen
    10. Beidong Liu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study sheds light on the role of sphingolipids on the maturation of Parkinson's disease-associated Synphilin-1 inclusion bodies (SY1 IBs) on the mitochondrial surface in a yeast model using Synthetic Genetic Array (SGA) and state-of-the-art imaging techniques. The authors provide solid evidence that downregulating the sphingolipid biosynthesis pathway leads to defective maturation and enhanced toxicity of SY1 IBs in both yeast and mammalian cells. However, these data neither explain the role of mitochondrial surface sphingolipids in SY1 IB maturation (the cellular site of maturation of smaller toxic aggregates to bigger less toxic IBs), nor the requirement of mitochondrial activity in IB maturation.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  6. Catalytic growth in a shared enzyme pool ensures robust control of centrosome size

    This article has 2 authors:
    1. Deb Sankar Banerjee
    2. Shiladitya Banerjee
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable work deals with mathematical modeling of centrosome maturation, building on the insight that autocatalytic assembly of the centrosome leads to size inequality. To remedy this, the authors propose a catalytic growth model with a shared enzyme pool that is able to reproduce various experimental results such as centrosome size scaling with cell size and centrosome growth curves in C. elegans. While finding the work of interest, the strength of the evidence presented in favor of the model is incomplete.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  7. TRIP13 localizes to synapsed chromosomes and functions as a dosage-sensitive regulator of meiosis

    This article has 7 authors:
    1. Jessica Y. Chotiner
    2. N. Adrian Leu
    3. Fang Yang
    4. Isabella G. Cossu
    5. Yongjuan Guan
    6. Huijuan Lin
    7. P. Jeremy Wang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study reports an important physiological function of a conserved meiosis factor in spermatogenesis in mice. The genetic and cell biological evidence supporting the conclusion is convincing. This work will be of broad interest to cell biologists, geneticists, and reproductive biologists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

    This article has 4 authors:
    1. Madeline M. McGinnis
    2. Benjamin M. Sutter
    3. Samira Jahangiri
    4. Benjamin P. Tu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study in budding yeast (S. cerevisiae) presents important findings demonstrating that the exonuclease Xrn1 regulates autophagy in response to methionine deprivation through effects on TORC1. There is solid evidence that the impact of Xrn1 on TORC1 is contingent on its catalytic activity rather than the degradation of any specific category of mRNAs. A major strength is the novel mechanism, in which Xrn1 modulates the nucleotide-binding state of the Gtr1/2 complex.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  9. Effect of alpha-tubulin acetylation on the doublet microtubule structure

    This article has 8 authors:
    1. Shun Kai Yang
    2. Shintaroh Kubo
    3. Corbin Black
    4. Katya Peri
    5. Daniel Dai
    6. Melissa Valente
    7. Jacek Gaertig
    8. Khanh Huy Bui
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In their fundamental study, the authors employ a combination of cryo-electron microscopy, molecular dynamics, and mass spectrometry to elucidate the impact of α-tubulin acetylation at the lumenal lysine 40 residue (αK40) on the structure and stability of doublet microtubules in cilia. While the work provides compelling evidence for the role of αK40 acetylation in the cilium, the current version could benefit from additional statistical analyses and clarification of its conclusions regarding the effects of acetylation on microtubule inner proteins (MIPs).

    Reviewed by eLife

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  10. The exocyst complex controls multiple events in the pathway of regulated exocytosis

    This article has 5 authors:
    1. Sofía Suárez Freire
    2. Sebastián Pérez-Pandolfo
    3. Sabrina M. Fresco
    4. Pablo Wappner
    5. Mariana Melani
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study makes a valuable contribution by characterizing the role of the exocyst in secretory granule exocytosis in the Drosophila larval salivary gland. The results lead to the novel interpretation that the exocyst participates not only in exocytosis, but also in earlier steps of secretory granule biogenesis and maturation. Although these ideas are potentially of interest to a wide range of membrane traffic researchers, the evidence is incomplete, and the authors are urged to consider the possibility that inactivation of an essential exocytosis component might have indirect effects on other parts of the secretory pathway.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  11. Senescence of endplate osteoclasts induces sensory innervation and spinal pain

    This article has 8 authors:
    1. Dayu Pan
    2. Kheiria Gamal Benkato
    3. Xuequan Han
    4. Jinjian Zheng
    5. Vijay Kumar
    6. Mei Wan
    7. Junying Zheng
    8. Xu Cao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental work by Cao et al. advances our understanding of the role of senescent osteoclasts (SnOCs) in the pathogenesis of spine instability. The authors provide compelling evidence for the SnOCs to induce sensory nerve innervation. Subsequently, reduction of SnOCs by the senolytic drug Navitoclax markedly reduces spinal pain sensitivity. This work will be of broad interest to regenerative biologists working on spinal pain.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  12. Genetic Screen Identified Prmt5 as a Neuroprotection Target against Cerebral Ischemia

    This article has 9 authors:
    1. Haoyang Wu
    2. Peiyuan Lv
    3. Jinyu Wang
    4. Brian Bennett
    5. Jiajia Wang
    6. Pishun Li
    7. Yi Peng
    8. Guang Hu
    9. Jiaji Lin
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors performed a useful RNAi screen to identify epigenetic regulators involved in oxygen-glucose deprivation (OGD)-induced neuronal injury. PRMT5 was identified as a negative regulator of neuronal cell survival after OGD. Solid in vitro and in vivo data suggest that PRMT5 could be a novel therapeutic target for the treatment of ischemic stroke.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  13. Neural network emulation of the human ventricular cardiomyocyte action potential: a tool for more efficient computation in pharmacological studies

    This article has 10 authors:
    1. Thomas Grandits
    2. Christoph M. Augustin
    3. Gundolf Haase
    4. Norbert Jost
    5. Gary R. Mirams
    6. Steven A. Niederer
    7. Gernot Plank
    8. András Varró
    9. László Virág
    10. Alexander Jung
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study reports on the potential of neural networks to emulate simulations of human ventricular cardiomyocyte action potentials for various ion channel parameters with the advantage of saving simulation time in certain conditions. The evidence supporting the claims of the authors is solid, although the inclusion of open analysis of drop-off accuracy and validation of the neural network emulators against experimental data would have strengthened the study. The work will be of interest to scientists working in cardiac simulation and quantitative pharmacology.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  14. Ex vivo Expansion Potential of Murine Hematopoietic Stem Cells: A Rare Property Only Partially Predicted by Phenotype

    This article has 5 authors:
    1. Qinyu Zhang
    2. Rasmus Olofzon
    3. Anna Konturek-Ciesla
    4. Ouyang Yuan
    5. David Bryder
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents valuable data on how HSCs are expanded under PVA cultures. The functional evidence supporting the claims of the authors is solid, although an extended multi-omic data analysis could have strengthened the study. The work will be of interest to individuals within this HSC field.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  15. Apoptosis recognition receptors regulate skin tissue repair in mice

    This article has 9 authors:
    1. Olivia Justynski
    2. Kate Bridges
    3. Will Krause
    4. Maria Fernanda Forni
    5. Quan Phan
    6. Teresa Sandoval-Schaefer
    7. Ryan Driskell
    8. Kathryn Miller-Jensen
    9. Valerie Horsley
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors studied the mechanisms by which dead cells are removed from the wounded skin in a process called efferocytosis. By analyzing different cell populations in the skin, the authors find that proteins involved in mediating the cell death and marking the cells as undergoing this process are elevated during distinct times in the wound healing program. Interestingly, these same proteins are elevated even higher in diabetic wounds. Finally the authors demonstrate that blocking the process of efferocytosis alters the wound healing program, thus illustrating its importance in effective wound repair.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  16. Distinct transcriptomic profile of satellite cells contributes to preservation of neuromuscular junctions in extraocular muscles of ALS mice

    This article has 7 authors:
    1. Ang Li
    2. Jianxun Yi
    3. Xuejun Li
    4. Li Dong
    5. Lyle W. Ostrow
    6. Jianjie Ma
    7. Jingsong Zhou
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript describes valuable information on how the extraocular muscles (EOM) are preserved in a mouse model of familial Amyotrophic lateral sclerosis (ALS) that carries a G93A mutation in the Sod1 gene. The authors provide convincing evidence of how the integrity of neuromuscular junction is preserved in EOM but not in limb and diaphragm muscles of G93A mice. Overall, this interesting work provides new evidence regarding the etiopathogenesis of ALS and insights for the development of therapeutic targets to slow the loss of neuromuscular function in ALS.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 3 listsLatest version Latest activity
  17. Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

    This article has 6 authors:
    1. Mathieu C. Husser
    2. Nhat P. Pham
    3. Chris Law
    4. Flavia R. B. Araujo
    5. Vincent J.J. Martin
    6. Alisa Piekny
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this study, the authors develop a useful strategy for fluorophore-tagging endogenous proteins in human induced pluripotent stem cells (iPSCs) using a split mNeonGreen approach. Experimentally, the methods are solid, and the data presented support the author's conclusions. Overall, these methodologies should be useful to a wide audience of cell biologists who want to study protein localization and dynamics at endogenous levels in iPSCs.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  18. Rab7 dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis

    This article has 15 authors:
    1. Preksha Gaur
    2. Yesheswini Rajendran
    3. Bhagyashree Srivastava
    4. Manasvini Markandey
    5. Vered Fishbain-Yoskovitz
    6. Gayatree Mohapatra
    7. Aamir Suhail
    8. Shikha Chaudhary
    9. Shaifali Tyagi
    10. Subhash C Yadav
    11. Amit K Pandey
    12. Yifat Merbl
    13. Avinash Bajaj
    14. Vineet Ahuja
    15. Chittur V Srikanth
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study provides important insights into the mechanisms underlying ulcerative colitis, a chronic and debilitating gastrointestinal condition. The article provides solid evidence of the role of the vesicular trafficking protein Rab7 in regulating the colonic mucus system and its implications in ulcerative colitis.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  19. Human receptive endometrial organoid for deciphering the implantation window

    This article has 15 authors:
    1. Yu Zhang
    2. Rusong Zhao
    3. Chaoyan Yang
    4. Jinzhu Song
    5. Peishu Liu
    6. Yan Li
    7. Boyang Liu
    8. Tao Li
    9. Changjian Yin
    10. Minghui Lu
    11. Zhenzhen Hou
    12. Chuanxin Zhang
    13. Zi-Jiang Chen
    14. Keliang Wu
    15. Han Zhao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents initial findings in the generation of 3D cell constructs from endometrial cell mixtures seeded in Matrigel scaffold and treated with hormones as a proof of concept. While the study findings are valuable, functional validation to demonstrate its robustness is lacking, and therefore the strength of evidence is incomplete. The term organoids might not be appropriate to describe this in vitro model.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  20. Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

    This article has 2 authors:
    1. Ibtisam Ibtisam
    2. Alexei F Kisselev
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents important findings on a transcription-independent component of the early recovery of proteasome activity from a short pulse of proteasome inhibitor treatment. While the evidence supporting this conclusion is solid, experimental support forthe proposal of an alternative regulatory process operative at the level of proteasome assembly, is incomplete. Lacking is experimental quantification of the effect of proteasome inhibition on the rate at which newly synthesized subunits are assembled into proteasomes.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity