Showing page 1 of 27 pages of list content

  1. Amoeboid cells undergo durotaxis with soft end polarized NMIIA

    This article has 9 authors:
    1. Chenlu Kang
    2. Pengcheng Chen
    3. Xin Yi
    4. Dong Li
    5. Yiping Hu
    6. Yihong Yang
    7. Huaqing Cai
    8. Bo Li
    9. Congying Wu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment:

      This study presents an important finding on durotaxis in various amoeboid cells that is independent of focal adhesions. The evidence supporting the authors' claims is compelling. The work will be of interest to cell biologists and biophysicists working on rigidity sensing, the cytoskeleton, and cell migration.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  2. A Drug Repurposing Approach Reveals Targetable Epigenetic Pathways in Plasmodium vivax Hypnozoites

    This article has 48 authors:
    1. S. P. Maher
    2. M. A. Bakowski
    3. A. Vantaux
    4. E. L. Flannery
    5. C. Andolina
    6. M. Gupta
    7. Y. Antonova-Koch
    8. M. Argomaniz
    9. M. Cabrera-Mora
    10. B. Campo
    11. A. T. Chao
    12. A. K. Chatterjee
    13. W. T. Cheng
    14. E. Chuenchob
    15. C. A. Cooper
    16. K. Cottier
    17. M. R. Galinski
    18. A. Harupa-Chung
    19. H. Ji
    20. S. B. Joseph
    21. T. Lenz
    22. S. Lonardi
    23. J. Matheson
    24. S. A. Mikolajczak
    25. T. Moeller
    26. A. Orban
    27. V. PadĂ­n-Irizarry
    28. K. Pan
    29. J. PĂ©neau
    30. J. Prudhomme
    31. C. Roesch
    32. A. A. Ruberto
    33. S. S. Sabnis
    34. C. L. Saney
    35. J. Sattabongkot
    36. S. Sereshki
    37. S. Suriyakan
    38. R. Ubalee
    39. Y. Wang
    40. P. Wasisakun
    41. J. Yin
    42. J. Popovici
    43. C. W. McNamara
    44. C. J. Joyner
    45. F. Nosten
    46. B. Witkowski
    47. K. G. Le Roch
    48. D. E. Kyle
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper reports a large drug repurposing screen based on an in vitro culture platform to identify compounds that can kill Plasmodium hypnozoites. This valuable work adds to the current repertoire of anti-hypnozoites agents and uncovers targetable epigenetic pathways to enhance our understanding of this mysterious stage of the Plasmodium life cycle. The data presented here are based on solid methodology and represent a starting point for further investigation of epigenetic inhibitors to treat P. vivax infection. This paper will be of interest to Plasmodium researchers and more broadly to readers in the fields of host-pathogen interactions and drug development.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. Dietary bacteria control C. elegans fat content through pathways converging at phosphatidylcholine

    This article has 13 authors:
    1. Hsiao-Fen Han
    2. Shao-Fu Nien
    3. Hang-Shiang Jiang
    4. Jui-Ching Wu
    5. Chia-Yi Chiang
    6. Man-Tzu Li
    7. Leng-Jie Huang
    8. Sufeng Chiang
    9. Lien-Chieh Lin
    10. Yi-Ting Chuang
    11. Yu-Ho Lin
    12. Chao-Wen Wang
    13. Yi-Chun Wu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This a useful study that reports a genetic regulatory network that accounts for altered lipid metabolism in response to two different bacterial diets of C. elegans. The proposed mechanism, linking vitamin B12, S-adenosyl methionine (SAM), phosphatidylcholine (PC), and neutral lipid levels, is solid but has been previously demonstrated by other studies using similar assays. The evidence to support a new layer of regulation, via the production of phospho-choline by ASM-3/acid sphingomyelinase, requires further substantiation.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  4. Engineered Migrasomes: A Robust, Thermally Stable Vaccination Platform

    This article has 11 authors:
    1. Dongju Wang
    2. Haifang Wang
    3. Wei Wan
    4. Zihui Zhu
    5. Takami Sho
    6. Yi Zheng
    7. Xing Zhang
    8. Longyu Dou
    9. Qiang Ding
    10. Li Yu
    11. Zhihua Liu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study, from the group that pioneered migrasome, describes a novel vaccine platform derived from this newly discovered organelle. Using these cleverly engineered migrasomes – that behave like natural migrasomes – as a novel vaccine platform has the potential to overcome obstacles such as cold chain issues for vaccines like messenger RNA. Although the findings are important with practical implications for the vaccine technology, and the evidence, based on appropriate and validated methodology is convincing and is in line with current state-of-the-art, there are some critical issues that need to be addressed. These include a head-to-head comparison with proven vaccine platforms, for example, a SARS-CoV-2 mRNA vaccine or an adjuvanted recombinant spike protein.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  5. Clearance of protein aggregates during cell division

    This article has 7 authors:
    1. Shoukang Du
    2. Yuhan Wang
    3. Bowen Chen
    4. Shuangshuang Xie
    5. Kuan Yoow Chan
    6. David C. Hay
    7. Ting Gang Chew
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      How misfolded proteins are segregated and cleared is a significant question in mechanistic cell biology, since clearance of these aggregates can protect against pathologies that may otherwise arise. The authors discover a cell cycle stage-dependent clearing mechanism that involves the ER chaperone BiP, the proteosome, and CDK inactivation, but is curiously independent of the APC. These are valuable and interesting new observations, but the evidence supporting these claims is incomplete, and needs to be strengthened and further validated.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. Microtubule-dependent orchestration of centriole amplification in brain multiciliated cells

    This article has 13 authors:
    1. Amélie-Rose Boudjema
    2. Rémi Balagué
    3. Cayla E Jewett
    4. Gina M LoMastro
    5. Olivier Mercey
    6. Adel Al Jord
    7. Marion Faucourt
    8. Alexandre Schaeffer
    9. Camille Noûs
    10. Nathalie Delgehyr
    11. Andrew J Holland
    12. Nathalie Spassky
    13. Alice Meunier
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this important study, Boudjema et al. use cell culture models and advanced microscopic imaging to provide detailed analyses of the cellular events underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. This largely descriptive work provides a better understanding of this process that is of interest to cell biologists studying centrioles and cilia. Most of the claims are supported by the data, but the study would benefit from additional analyses regarding the roles of microtubules, which are currently incomplete, and from text editing to improve accessibility and readability, especially for a wider audience.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  7. Genome concentration limits cell growth and modulates proteome composition in Escherichia coli

    This article has 9 authors:
    1. Jarno Mäkelä
    2. Alexandros Papagiannakis
    3. Wei-Hsiang Lin
    4. Michael C. Lanz
    5. Skye Glenn
    6. Matthew Swaffer
    7. Georgi K. Marinov
    8. Jan M. Skotheim
    9. Christine Jacobs-Wagner
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study represents a fundamental contribution to our understanding of how gene expression levels are controlled in bacteria. Through a series of compelling and careful experiments, relying on a mutant that blocks DNA replication but permits growth, and using various methods, the authors reveal how genome concentration rapidly becomes limiting for growth when replication is inhibited. This work contributes to our understanding of the contributions and limiting roles of DNA, mRNA, and ribosomes for growth in bacteria, and will be of considerable interest within both systems biology and microbial physiology.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  8. A genome wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6⍺

    This article has 6 authors:
    1. Joanne Tung
    2. Lei Huang
    3. Ginto George
    4. Heather P. Harding
    5. David Ron
    6. Adriana Ordoñez
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors explore ER stress signalling mediated by ATF6 using a genome-wide gene depletion screen. They find that the ER chaperone Calreticulin binds and directly represses ATF6; this proposed function for Calreticulin is intriguing and constitutes an important finding. The evidence presented is based on CHO genetic evidence and biochemical results and is convincing.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  9. Novel 3D Approach to Model Non-Alcoholic Fatty Liver Disease using human Pluripotent Stem Cells

    This article has 18 authors:
    1. Carola Maria Morell
    2. Samantha Grace Tilson
    3. Rute Alexandra Tomaz
    4. Arash Shahsavari
    5. Andi Munteanu
    6. Giovanni Canu
    7. Brandon Tyler Wesley
    8. Marion Perrin
    9. Imbisaat Geti
    10. Subhankar Mukhopadhyay
    11. Francesca Mazzacuva
    12. Paul Gissen
    13. Jose Garcia-Bernardo
    14. Martin Bachman
    15. Casey Allison Rimland
    16. Fotios Sampaziotis
    17. Irina Mohorianu
    18. Ludovic Vallier
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors present an important study of a multi-cellular platform involving co-culturing of various hiPSC-derived hepatocyte like cells, cholangiocytes, stellate cells and macrophages to mimic the liver microenvironment. The aggregates are then treated with fatty acids and examined through transcriptomic and functional assays. The techniques and methodologically are sound, and the evidence supporting the conclusion is convincing, although more clinically relevant data demonstrating the effect of some potential pharmacological agents on the platform would serve to strengthen the study.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  10. Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

    This article has 19 authors:
    1. Xiaoning Gao
    2. WuYan Guo
    3. Peiyuan Liu
    4. Mingyue Yuwen
    5. Zixiang Liu
    6. Ruyang Tan
    7. Kairui Liu
    8. Zhiru Yang
    9. Junli Ba
    10. Xue Bai
    11. Shiti Shama
    12. Cong Tang
    13. Kai Miao
    14. Haozhi Pei
    15. Liren Liu
    16. Cheng Zhu
    17. Tao Wang
    18. Bo Zhang
    19. Jun Kang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study suggests that capsaicin nanoparticle administration in rats activates the transcription factor Nrf2 by directly binding to its repressor KEAP1, leading to cytoprotective gene induction, and preventing alcohol-induced gastric damage, an avenue to treat alcoholism-related gastric disorders. The evidence is currently incomplete as there is no experimental proof that capsaicin exerts its cytoprotective effects via Nrf2, and not via any of its multiple known pharmacological effects. In particular, Nrf2-deficient mice should be used to show that Nrf2 is causal to the cytoprotective effect, and better controls should be provided for the direct KEAP2-capsaicin interaction, given the high concentrations used.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  11. The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

    This article has 14 authors:
    1. Fabian Link
    2. Sisco Jung
    3. Xenia Malzer
    4. Felix Zierhut
    5. Antonia Konle
    6. Alyssa Borges
    7. Christopher Batters
    8. Monika Weiland
    9. Mara Pöllmann
    10. An Binh Nguyen
    11. Johannes Kullmann
    12. Claudia Veigel
    13. Markus Engstler
    14. Brooke Morriswood
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study builds on a previous publication (with partially overlapping authors), demonstrating that T. brucei has a continuous endomembrane system, which probably facilitates high rates of endocytosis. Using a range of cutting-edge approaches, the authors present compelling evidence that an actomyosin system, with the myosin TbMyo1 as the molecular motor, is localized close to the endosomal system in the bloodstream form (BSF) of Trypanosoma brucei. It shows convincingly that actin plays a role in the organization and integrity of the endosomal system, and that the trypanosome Myo1is an active motor that interacts with actin and transiently associates with endosomes, but a role of Myo1 in endomembrane function in vivo was not directly demonstrated. This work should be of interest to cell biologists and microbiologists working on the cytoskeleton, and unicellular eukaryotes.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  12. Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

    This article has 5 authors:
    1. Senem Ntourmas
    2. Martin Sachs
    3. Martina BrĂĽckner
    4. JĂĽrgen Behrens
    5. Dominic B. Bernkopf
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behavior and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study is, however, incomplete due to the lack of several controls and its focus on overexpression and mutants lacking key domains.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  13. BEND2 is a crucial player in oogenesis and reproductive aging

    This article has 11 authors:
    1. Yan Huang
    2. Nina Bucevic
    3. Carmen Coves
    4. Natalia Felipe-Medina
    5. Marina Marcet-Ortega
    6. Nikoleta Nikou
    7. Cristina Madrid-SandĂ­n
    8. Neus Ferrer Miralles
    9. Antoni Iborra
    10. Alberto M. Pendás
    11. Ignasi Roig
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides valuable information on a novel gene that regulates meiotic progression in both male and female meiosis, but the evidence supporting the conclusions of the authors on the role of BEND2 in oogenesis and reproductive aging is incomplete. This study will be of interest to developmental and reproductive biologists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  14. A novel human pluripotent stem cell-based gene activation system identifies IGFBP2 as a mediator in the production of hematopoietic progenitors in vitro

    This article has 11 authors:
    1. Paolo Petazzi
    2. Telma Ventura
    3. Francesca Paola Luongo
    4. Heather McClafferty
    5. Alisha May
    6. Helen Alice Taylor
    7. Micheal J Shipston
    8. Nicola Romanò
    9. Lesley M. Forrester
    10. Pablo Menéndez
    11. Antonella Fidanza
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents useful findings to inform and improve the in vitro differentiation of hematopoietic progenitor cells from human induced pluripotent stem cells. However, while relying on a well-characterized technical approach, the data analysis is overall incomplete and only partially supports the main conclusions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  15. The sperm hook in house mice: a functional adaptation for migration and self-organised behaviour

    This article has 9 authors:
    1. Heungjin Ryu
    2. Kibum Nam
    3. Byeong Eun Lee
    4. Yundon Jeong
    5. Seunghun Lee
    6. Jeongmo Kim
    7. Young-Min Hyun
    8. Jae-Ick Kim
    9. Jung-Hoon Park
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study uses ex vivo live imaging of the uterus, uterotubal junction, and oviduct post-mating to test the role of the sperm hook in the house mouse (Mus musculus) in sperm movement which could be interesting to evolutionary biologists. The work is useful as their live imaging revealed sperm behaviors in the female tract that have not been previously reported. However, the strength of evidence is incomplete since the limited quantification of the data is insufficient and the extensive speculation on the functions of these sperm behaviors is not supported by sufficient experimental evidence to support their conclusions.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  16. Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistance

    This article has 6 authors:
    1. Jeongsik Kim
    2. Dahyun Kim
    3. Dong-Kyun Kim
    4. Sang-Hee Lee
    5. Wonyul Jang
    6. Dae-Sik Lim
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study reports the formation of a new organelle, called giant unilocular vacuole (GUVac), in mammary epithelial cells through a macropinocytosis-like process. The evidence supporting conclusions is solid, using state-of-the-art cell biology techniques. This work will be of interest to cell biologists and contribute to the understanding of cell survival mechanisms against anoikis.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  17. Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

    This article has 10 authors:
    1. Yue Miao
    2. Yongtao Du
    3. Baolei Wang
    4. Jingjing Liang
    5. Yu Liang
    6. Song Dang
    7. Jiahao Liu
    8. Dong Li
    9. Kangmin He
    10. Mei Ding
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The manuscript presents a useful model for the field of endosome maturation, providing perspective on the role of the deubiquitinating enzyme UPS-50/USP8 in the process. The evidence presented in the paper is clear, incorporating well-designed experiments that suggest the dual actions of UPS-50 and USP8 in the conversion of early endosomes into late endosomes. Overall, the work is solid and centers on an intriguing subject.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  18. Focal adhesion-derived liquid-liquid phase separations regulate mRNA translation

    This article has 3 authors:
    1. Abhishek Kumar
    2. Keiichiro Tanaka
    3. Martin A Schwartz
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this valuable study, Kumar et al., provide evidence suggesting that the p130Cas drives the formation of condensates that sprout from focal adhesions to cytoplasm and suppress translation. Pending further substantiation, this study was found to be likely to provide previously unappreciated insights into the mechanisms linking focal adhesions to the regulation of protein synthesis and was thus considered to be of broad general interest. However, the evidence supporting the proposed model was incomplete; additional evidence is warranted to substantiate the relationship between p130Cas condensates and mRNA translation and establish corresponding functional consequences.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  19. Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

    This article has 8 authors:
    1. Weihua Wang
    2. Junqiao Xing
    3. Xiqi Zhang
    4. Hongni Liu
    5. Haochen Jiang
    6. Cheng Xu
    7. Xue Zhao
    8. Zhangfeng Hu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study reports useful data suggesting the critical roles of two ancient proteins, XAP5 and XAP5L, in controlling the transcriptional program of ciliogenesis during mouse spermatogenesis. However, this study is considered incomplete because the data only partially support the conclusion. This work will be of interest to biomedical researchers who work on ciliogenesis and reproduction.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  20. Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach

    This article has 12 authors:
    1. Kourosh Hayatigolkhatmi
    2. Chiara Soriani
    3. Emanuel Soda
    4. Elena Ceccacci
    5. Oualid El Menna
    6. Sebastiano Peri
    7. Ivan Negrelli
    8. Giacomo Bertolini
    9. Gian Martino Franchi
    10. Roberta Carbone
    11. Saverio Minucci
    12. Simona Rodighiero
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper presents a valuable automated method to track individual mammalian cells as they progress through the cell cycle using the FUCCI system. The authors have developed a technique for analyzing cells that grow in suspension and used their method to look at different tumor cell lines that grow in suspension and determine the effect of drugs that directly affect the cell cycle. They show solid evidence that the method can be applied to both adherent and non-adherent cell lines. This paper will be of interest to cell biologists investigating cell cycle effects.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity