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  1. A hepatocyte-specific transcriptional program driven by Rela and Stat3 exacerbates experimental colitis in mice by modulating bile synthesis

    This article has 12 authors:
    1. Jyotsna Singh
    2. Binayak Sarkar
    3. Mohit Yadav
    4. Alvina Deka
    5. Manasvini Markandey
    6. Priyadarshini Sanyal
    7. Perumal Nagarajan
    8. Nilesh Gaikward
    9. Vineet Ahuja
    10. Debasisa Mohanty
    11. Soumen Basak
    12. Rajesh S Gokhale
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study reveals the RelA/Stat3-dependent gene program in the liver influences intestinal homeostasis. The evidence supporting the conclusions is compelling, although some additional experiments will strengthen the study. The work will be of interest to scientists in gastrointestinal research fields.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. G protein subunit G γ 13 -mediated signaling pathway is critical to the inflammation resolution and functional recovery of severely injured lungs

    This article has 9 authors:
    1. Yi-Hong Li
    2. Yi-Sen Yang
    3. Yan-Bo Xue
    4. Hao Lei
    5. Sai-Sai Zhang
    6. Junbin Qian
    7. Yushi Yao
    8. Ruhong Zhou
    9. Liquan Huang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This in principle useful study suggests that the G-protein subunit Gng13 is required for limiting injury and inflammation following H1N1 influenza infection via anti-inflammatory effects from ectopic tuft cells. There appears to be support for Gng13 helping to limit influenza injury in the transgenic mouse models used here, but evidence for these effects being mediated by tuft cells is incomplete, giving conflicting data from mice that lack tuft cells entirely.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  3. Developmental conversion of thymocyte-attracting cells into self-antigen-displaying cells in embryonic thymus medulla epithelium

    This article has 12 authors:
    1. Izumi Ohigashi
    2. Andrea White
    3. Mei-Ting Yang
    4. Sayumi Fujimori
    5. Yu Tanaka
    6. Alison Jacques
    7. Hiroshi Kiyonari
    8. Yosuke Matsushita
    9. Sevilay Turan
    10. Michael C Kelly
    11. Graham Anderson
    12. Yousuke Takahama
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is an important study that provides new insights into the development and function of medullary thymus epithelial cells (mTEC). The authors provide compelling evidence to support their claims as to the differentiation and lineage outcomes of CCL21+ mTEC progenitors, which further our understanding of how central tolerance of T cells is enforced within the thymus.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  4. WASP facilitates tumor mechanosensitivity in T lymphocytes

    This article has 10 authors:
    1. Srishti Mandal
    2. Mariane Melo
    3. Pavlo Gordiichuk
    4. Sayanti Acharya
    5. Yeh-Chuin Poh
    6. Na Li
    7. Aereas Aung
    8. Eric L. Dane
    9. Darrell J. Irvine
    10. Sudha Kumari
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study employs a diverse array of techniques encompassing cell biological manipulations, biophysical measurements, and mouse models to elucidate the impact of target cell stiffness on CD8+ cytotoxic T cell activation, with a particular focus on the actin nucleator protein WASP. The finding that WASP is essential for the stiffness-dependent phosphorylation of ZAP70 in CD8 T cells is convincing. However, the data regarding the role of WASP in mechanosensing within CD8 T cell-mediated anti-tumor immunity is incomplete and would benefit from a more rigorous study design. This work would be of interest to cell biologists and investigators studying mechanosensing within the immune system.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  5. A novel MARV glycoprotein-specific antibody with potentials of broad-spectrum neutralization to filovirus

    This article has 18 authors:
    1. Yuting Zhang
    2. Min Zhang
    3. Haiyan Wu
    4. Xinwei Wang
    5. Hang Zheng
    6. Junjuan Feng
    7. Jing Wang
    8. Longlong Luo
    9. He Xiao
    10. Chunxia Qiao
    11. Xinying Li
    12. Yuanqiang Zheng
    13. Weijin Huang
    14. Youchun Wang
    15. Yi Wang
    16. Yanchun Shi
    17. Jiannan Feng
    18. Guojiang Chen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this valuable study, the discovery and subsequent design of the AF03-NL chimeric antibody yielded a tool for studying filoviruses and provides a possible blueprint for future therapeutics. However, the data are incomplete and not presented clearly, which obscures flaws in the analyses and leaves unexplained phenomena. The work will be of interest to virologists studying antibodies.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  6. Caspase-1 activates gasdermin A in non-mammals

    This article has 6 authors:
    1. Zachary P. Billman
    2. Stephen B. Kovacs
    3. Bo Wei
    4. Kidong Kang
    5. Ousmane H. Cissé
    6. Edward A. Miao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents valuable insights into the evolution of the gasdermin family, making a strong case that a GSDMA-like gasdermin was already present in early land vertebrates and was activated by caspase-1 cleavage. Convincing biochemical evidence is provided that extant avian, reptile, and amphibian GSDMA proteins can still be activated by caspase-1 and upon cleavage induce pyroptosis-like cell death - at least in human cell lines. The caspase-1 cleavage site is only lost in mammals, which use the more recently evolved GSDMD as a caspase-1 cleavable pyroptosis inducer. The presented work will be of considerable interest to scientists working on the evolution of cell death pathways, or on cell death regulation in non-mammalian vertebrates.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  7. MARCO in alveolar macrophages negatively regulates Ace expression and aldosterone production

    This article has 6 authors:
    1. Conan J O O’Brien
    2. Giorgio Ratti
    3. Emma Haberman
    4. Charles Sweeney
    5. Siamon Gordon
    6. Ana I Domingos
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      O'Brien and co-authors addressed how statins reduce levels of aldosterone in humans and provide important data demonstrating that tissue-resident macrophages can exert physiological functions and influence endocrine systems. However, the strength of evidence, as of now, is incomplete, as the sole description of the phenotype of MARCO-deficient mice is insufficient to claim that MARCO in alveolar macrophages can negatively regulate ACE expression and aldosterone production at steady-state. The work will be of broad interest to cell biologists and immunologists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. Foxp3 depends on Ikaros for control of regulatory T cell gene expression and function

    This article has 6 authors:
    1. Rajan M. Thomas
    2. Matthew C. Pahl
    3. Liqing Wang
    4. Struan F. A. Grant
    5. Wayne W. Hancock
    6. Andrew D. Wells
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This comprehensive study provides valuable information on the cooperation of Ikaros with Foxp3 to establish and regulate a major portion of the epigenome and transcriptome of T-regulatory cells. However, the characterization is incomplete in that incontrovertible evidence that these are intrinsic features regulating biological function and not outcomes of the inflammatory micro-environment of the genetically manipulated mice is missing.

    Reviewed by eLife

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  9. Comprehensive analysis of nasal IgA antibodies induced by intranasal administration of the SARS-CoV-2 spike protein

    This article has 9 authors:
    1. Kentarou Waki
    2. Hideki Tani
    3. Yumiko Saga
    4. Takahisa Shimada
    5. Emiko Yamazaki
    6. Seiichi Koike
    7. Mana Okada
    8. Masaharu Isobe
    9. Nobuyuki Kurosawa
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work provides valuable insights into mucosal antibody responses against SARS-CoV-2 following intranasal immunization by characterizing a large number of monoclonal antibodies at both mucosal and non-mucosal sites. The evidence supporting the claims is overall solid, although the flow cytometric assessment of antibody-expressing cells would benefit from more rigorous controls. The demonstrated in vitro antiviral activity of antibodies characterized provides a rationale for developing mucosal vaccines, especially if confirmed in vivo and benchmarked against antibodies generated following intramuscular vaccination.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  10. Noncanonical inflammasome assembly requires caspase-11 catalytic activity and intra-molecular autoprocessing

    This article has 6 authors:
    1. Daniel C. Akuma
    2. Daniel Grubaugh
    3. Chukwuma E. Odunze
    4. Sunny Shin
    5. Cornelius Y. Taabazuing
    6. Igor E. Brodsky
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Brodsky and colleagues report here an unexpected cis-activation mechanism of caspase-11. The authors use cellular imaging methods and cleavage site mutants to show that the LPS-induced speck formation by caspase-11 depends on the autoprocessing between two subdomains. This new finding opens multiple doors for further investigating how this non-canonical inflammasome is regulated and activated at the molecular level.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  11. Transposable elements regulate thymus development and function

    This article has 17 authors:
    1. Jean-David Larouche
    2. Céline M. Laumont
    3. Assya Trofimov
    4. Krystel Vincent
    5. Leslie Hesnard
    6. Sylvie Brochu
    7. Caroline Côté
    8. Juliette Humeau
    9. Éric Bonneil
    10. Joël Lanoix
    11. Chantal Durette
    12. Patrick Gendron
    13. Jean-Philippe Laverdure
    14. Ellen R. Richie
    15. Sébastien Lemieux
    16. Pierre Thibault
    17. Claude Perreault
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study shows, based on analyses of single cell RNA-seq data sets of thymus cells, that transposable elements (TEs) are broadly expressed in thymic stromal cells, especially in medullary thymic epithelial cells and plasamacytoid dendritic cells. The authors also show that at least some TE-derived peptides are presented by MHC-I molecules in the thymus. The results suggest a possible role of TEs in thymic T-cell selection and immune self-tolerance, but the current analyses are incomplete and not yet fully support the claims.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  12. Transposable elements regulate thymus development and function

    This article has 17 authors:
    1. Jean-David Larouche
    2. Céline M. Laumont
    3. Assya Trofimov
    4. Krystel Vincent
    5. Leslie Hesnard
    6. Sylvie Brochu
    7. Caroline Côté
    8. Juliette Humeau
    9. Éric Bonneil
    10. Joël Lanoix
    11. Chantal Durette
    12. Patrick Gendron
    13. Jean-Philippe Laverdure
    14. Ellen R. Richie
    15. Sébastien Lemieux
    16. Pierre Thibault
    17. Claude Perreault
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study shows, based on analyses of single cell RNA-seq data sets of thymus cells, that transposable elements (TEs) are broadly expressed in thymic stromal cells, especially in medullary thymic epithelial cells and plasamacytoid dendritic cells. The authors also show that at least some TE-derived peptides are presented by MHC-I molecules in the thymus. The results suggest a possible role of TEs in thymic T-cell selection and immune self-tolerance, but the current analyses are incomplete and not yet fully support the claims.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  13. RAG1 and RAG2 non-core regions are implicated in the leukemogenesis and off-target V(D)J recombination in BCR-ABL1 -driven B cell lineage lymphoblastic leukemia

    This article has 10 authors:
    1. Xiaozhuo Yu
    2. Wen Zhou
    3. Xiaodong Chen
    4. Shunyu He
    5. Mengting Qin
    6. Meng Yuan
    7. Yang Wang
    8. Woodvine otieno Odhiambo
    9. Yinsha Miao
    10. Yanhong Ji
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors have shown a valuable tumor suppressive function of the non-core regions of RAG1/2 recombinases, by using a set of animal models. The work is solid and the conclusions are supported by their data. Some areas of mechanistic work can be improved.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  14. Stimulation-induced cytokine polyfunctionality as a dynamic concept

    This article has 3 authors:
    1. Kevin Portmann
    2. Aline Linder
    3. Klaus Eyer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is a useful study that uses a microfluidic method to evaluate the ability of single human white blood cells to produce combinations of cytokines. The evidence that this takes place is solid. The paper highlights polyfunctionality using similar data to a prior work from the same group.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  15. H2-O deficiency promotes regulatory T cell differentiation and CD4 T cell hyperactivity

    This article has 5 authors:
    1. Robin A. Welsh
    2. Nianbin Song
    3. Chan-su Park
    4. J. David Peske
    5. Scheherazade Sadegh-Nasseri
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper seeks to understand how the presentation of peptides by medullary thymic epithelial cells may be regulated by the MHCII peptide loading modulator, H2-O, and how this may affect the selection of regulatory T (Treg) cells. Further work is needed to ensure that the findings are robust: currently the analysis of data is inadequate and inconsistencies in the reported findings are not placed in context with results from other groups. The current version does not provide sufficient support for the claims regarding the effects on Treg cell selection.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  16. Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasome-induced blood coagulation and inflammation

    This article has 10 authors:
    1. Jian Cui
    2. Hua Li
    3. Guoying Zhang
    4. Yan Zhang
    5. Ling Yang
    6. Martha M.S. Sim
    7. Jeremy P. Wood
    8. Yinan Wei
    9. Zhenyu Li
    10. Congqing Wu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors aim to elucidate the mechanism by which pyroptosis contributes to the increased release of procoagulant tissue factor-containing microvesicles. The data are intriguing and attempt to shed light on the mechanism by which GSDMD plays a role in releasing tissue factor-containing microvesicles, albeit incomplete. The manuscript offers valuable information to unveil new therapeutic targets in human diseases such as sepsis, which can be further strengthened by consulting the reviewers' suggestions.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  17. Regulation of pDC Fate Determination by Histone Deacetylase 3

    This article has 7 authors:
    1. Yijun Zhang
    2. Zhimin He
    3. Wenlong Lai
    4. Xiangyi Shen
    5. Tao Wu
    6. Jiaoyan Lv
    7. Li Wu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study examines the expression of HDAC3 within DC compartment. Taking advantage of tamoxifen inducible ERT2-cre mouse model they observe the dependency of pDCs but not cDCs on HDAC3. The requirement of this histone modifier appears to occur during development around the CLP stage. Tamoxifen treated mice lack almost all pDC besides lymphoid progenitors. RNA seq studies identify multiple DC specific target genes within the remaining pDC - using Cut and Tag technology they validate some of the identified targets of HDAC3. Taken together, this study shows the requirement of HDAC3 on pDC but not cDC, congruent with the recent findings of a lymphoid origin of pDC.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  18. Piezo1 mechanosensing regulates integrin-dependent chemotactic migration in human T cells

    This article has 11 authors:
    1. Chinky Shiu Chen Liu
    2. Tithi Mandal
    3. Parijat Biswas
    4. Md. Asmaul Hoque
    5. Purbita Bandopadhyay
    6. Bishnu Prasad Sinha
    7. Jafar Sarif
    8. Ranit D’Rozario
    9. Deepak Kumar Sinha
    10. Bidisha Sinha
    11. Dipyaman Ganguly
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides useful insights into the subcellular localization, interaction with integrins, and function of the cell surface receptor Piezo1 in migrating human T-cells. The data collected is convincing but incomplete. Therefore the idea that Piezo1 is critically sensing mechano-physical cues during T-cell migration is not well supported by direct experimental evidence.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  19. Early acquisition of S-specific Tfh clonotypes after SARS-CoV-2 vaccination is associated with the longevity of anti-S antibodies

    This article has 7 authors:
    1. Xiuyuan Lu
    2. Hiroki Hayashi
    3. Eri Ishikawa
    4. Yukiko Takeuchi
    5. Julian Vincent Tabora Dychiao
    6. Hironori Nakagami
    7. Sho Yamasaki
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable finding on the key factors of T cell responses associated with durable antibody responses following COVID-19 mRNA vaccinations. The data were collected with solid methods and approaches, but the interpretation of the conclusion may be biased due to the experimental design. If confirmed, it may have a great impact on future COVID-19 vaccine design. However, some of the evidence supporting the claims of the authors is incomplete with a small size of the samples, unproven validity of using the expanded T cells, and over-interpretation on the sequence homology data.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  20. The antigenic landscape of human influenza N2 neuraminidases from 2009 until 2017

    This article has 19 authors:
    1. João Paulo Portela Catani
    2. Anouk Smet
    3. Tine Ysenbaert
    4. Marnik Vuylsteke
    5. Guy Bottu
    6. Janick Mathys
    7. Alexander Botzki
    8. Guadalupe Cortes-Garcia
    9. Tod Strugnell
    10. Raul Gomila
    11. John Hamberger
    12. John Catalan
    13. Irina V. Ustyugova
    14. Timothy Farrell
    15. Svetlana Stegalkina
    16. Satyajit Ray
    17. Lauren LaRue
    18. Xavier Saelens
    19. Thorsten U. Vogel
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents valuable data on the antigenic properties of neuraminidase proteins of human A/H3N2 influenza viruses sampled between 2009 and 2017. The antigenic properties are found to be generally concordant with genetic groups. However, the evidence for claims concerning the molecular basis of the antigenic differences remains incomplete, as the computational methods have been insufficiently validated.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity