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  1. Clinical phenotypes in acute and chronic infarction explained through human ventricular electromechanical modelling and simulations

    This article has 9 authors:
    1. Xin Zhou
    2. Zhinuo Jenny Wang
    3. Julia Camps
    4. Jakub Tomek
    5. Alfonso Santiago
    6. Adria Quintanas
    7. Mariano Vazquez
    8. Marmar Vaseghi
    9. Blanca Rodriguez
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study contributes to the current knowledge in the field of acute and chronic infarction. It is a significant study because the results provide convincing evidence for the need to incorporate additional risk factors for assessing patients after myocardial infarction.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  2. Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation

    This article has 42 authors:
    1. Megan M. Sperry
    2. Berenice Charrez
    3. Haleh Fotowat
    4. Erica Gardner
    5. Kanoelani Pilobello
    6. Zohreh Izadifar
    7. Tiffany Lin
    8. Abigail Kuelker
    9. Sahith Kaki
    10. Michael Lewandowski
    11. Shanda Lightbown
    12. Ramses Martinez
    13. Susan Marquez
    14. Joel Moore
    15. Maria Plaza-Oliver
    16. Adama M. Sesay
    17. Kostyantyn Shcherbina
    18. Katherine Sheehan
    19. Takako Takeda
    20. Daniela Del Campo
    21. Kristina Andrijauskaite
    22. Exal Cisneros
    23. Riley Lopez
    24. Isabella Cano
    25. Zachary Maxwell
    26. Israel Jessop
    27. Rafael J. Veraza
    28. Leon Bunegin
    29. Thomas J. Percival
    30. Jaclyn Yracheta
    31. Jorge Pena
    32. Diandra Wood
    33. Zachary Homas
    34. Cody Hinshaw
    35. Jennifer Cox-Hinshaw
    36. Olivia G. Parry
    37. Justin J. Sleeter
    38. Erik K. Weitzel
    39. Michael Levin
    40. Michael Super
    41. Richard Novak
    42. Donald E. Ingber
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study provides a new way to enhance organ preservation. The authors provide solid evidence that an existing drug, SNC80, can rapidly and reversibly slow biochemical and metabolic activities while preserving cell and tissue viability. This study will be of interest to a broad set of readers interested in organ transplantation, tissue engineering, regenerative medicine organoids, and organ-on-a-chip engineering.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. The vascularised chamber device significantly enhances the survival of transplanted liver organoids

    This article has 8 authors:
    1. Denis D. Shi
    2. Evelyn Makris
    3. Yi-Wen Gerrand
    4. Pu-Han Lo
    5. George C. Yeoh
    6. Wayne A. Morrison
    7. Geraldine M. Mitchell
    8. Kiryu K. Yap
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This solid manuscript describes a preclinical model to assess different methods of infusion of organoids for clinical applications. This is an important and timely study with practical implications beyond a single subfield. The methods described, including the analysis, broadly support the claims although there are some areas for improvement.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  4. Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

    This article has 5 authors:
    1. Fang Kong
    2. Megha Upadya
    3. Andrew See Weng Wong
    4. Rinkoo Dalan
    5. Ming Dao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work provides a robust yet simple protocol to isolate small extracellular vesicles from small volumes of plasma. The evidence supporting the conclusions is convincing, although a more thorough statistical comparison of the different techniques and technique combinations explored in the study would have been appreciated. The work will be of broad interest to cell biologists and biochemists.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  5. Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

    This article has 17 authors:
    1. Mami Matsuo-Takasaki
    2. Sho Kambayashi
    3. Yasuko Hemmi
    4. Tamami Wakabayashi
    5. Tomoya Shimizu
    6. Yuri An
    7. Kazuhiro Takeuchi
    8. Masato Ibuki
    9. Yoshikazu Kawai
    10. Masafumi Umekage
    11. Tomoaki M Kato
    12. Michiya Noguchi
    13. Koji Nakade
    14. Yukio Nakamura
    15. Tomoyuki Nakaishi
    16. Masayoshi Tsukahara
    17. Yohei Hayashi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors have identified a media condition that maintains iPSCs in suspension cultures by inhibiting the PKCβ and Wnt signaling pathways. The manuscript is valuable for the pluripotent stem cell field. This is a solid study with substantial data to support the conclusions in the manuscript.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. A three filament mechanistic model of musculotendon force and impedance

    This article has 3 authors:
    1. Matthew Millard
    2. David W. Franklin
    3. Walter Herzog
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is an important study that develops a new model of the way muscle responds to perturbations, synthesizing models of how it responds to small and large perturbations, both of which are important to predict how muscles function for stability but also how they can be injured. The evidence presented to support the model is solid, but the work is incomplete as it is particularly lacking a more detailed analysis of the trade-offs associated with using the model to simulate different types of preparations, especially single muscle fibers compared to whole muscles or whole muscles and tendons. With a clearer discussion of the limitations of the model and the situations in which it is best applied, the work will be of interest to those developing realistic models of the stability and control of movement in humans or other animals.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  7. Simple decoding of behavior from a complicated neural manifold

    This article has 4 authors:
    1. Sean M. Perkins
    2. John P. Cunningham
    3. Qi Wang
    4. Mark M. Churchland
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper presents a new method called MINT that is simple yet effective at BCI-style decoding tasks in stereotyped settings. While the reviewers raise caveats, overall they believe the work is a valuable study for the field of motor control, and the evidence to support their claims is solid.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. Near-perfect precise on-target editing of human hematopoietic stem and progenitor cells

    This article has 7 authors:
    1. Fanny-MeĂŻ Cloarec-Ung
    2. Jamie Beaulieu
    3. Arunan Suthananthan
    4. Bernhard Lehnertz
    5. Guy Sauvageau
    6. Hilary M Sheppard
    7. David JHF Knapp
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable methodology with increased efficiency and precision of gene editing in human primary hematopoietic stem and progenitor cells. The evidence supporting the claims is solid, although a more extensive assessment of functional hematopoietic stem cell impacts and potential off-target effects would be helpful to further strengthen the conclusions. The work will be of interest to biologists studying hematopoietic stem and progenitor cells and gene targeting for potential clinical applications.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  9. Overcoming the nutritional immunity by engineering iron scavenging bacteria for cancer therapy

    This article has 8 authors:
    1. Sin-Wei Huang
    2. See-Khai Lim
    3. Yao-An Yu
    4. Yi-Chung Pan
    5. Wan-Ju Lien
    6. Chung-Yuan Mou
    7. Che-Ming Jack Hu
    8. Kurt Yun Mou
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work is valuable in exploring the role of nutritional immunity in bacteria-mediated tumor therapeutics, involving proteomics and in vivo mouse model results which provide largely solid supporting evidence of the observational claims, but is incomplete when extrapolating the mechanisms of how manipulation of iron status can affect E. coli-mediated tumor therapy. This work is of interest to a broad audience including researchers in cancer biology, cell biology, and microbiology.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  10. Targeting sex determination to suppress mosquito populations

    This article has 15 authors:
    1. Ming Li
    2. Nikolay P Kandul
    3. Ruichen Sun
    4. Ting Yang
    5. Elena D Benetta
    6. Daniel J Brogan
    7. Igor Antoshechkin
    8. Héctor M Sánchez C
    9. Yinpeng Zhan
    10. Nicolas A DeBeaubien
    11. YuMin M Loh
    12. Matthew P Su
    13. Craig Montell
    14. John M Marshall
    15. Omar S Akbari
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable paper builds on a method, previously conceptualized and validated, of genetic control for insect populations. The method, called pgSIT, uses integrated CRISPR-Cas9 based constructs to generate, in certain combinations of genotypes, mutations that cause both male sterility and female inviability. Release of such genotypes in sufficiently large numbers can lead to an inundation of a local insect population with sterile males and this can lead to localised population suppression, which represents an effective method of control for problematic insect populations. The data are convincing and will be of interest to anyone working on vector control strategies.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 2 listsLatest version Latest activity
  11. An in vitro model for vitamin A transport across the human blood–brain barrier

    This article has 2 authors:
    1. Chandler B Est
    2. Regina M Murphy
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental work substantially advances our understanding of retinol transport through the blood-brain barrier. The evidence supporting the conclusions is compelling, with rigorous biochemical assays. In general, the work is of broad interest to cell biologists, biochemists and neuroscientists.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  12. Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors

    This article has 22 authors:
    1. Lampros Mavrommatis
    2. Hyun-Woo Jeong
    3. Urs Kindler
    4. Gemma Gomez-Giro
    5. Marie-Cecile Kienitz
    6. Martin Stehling
    7. Olympia E Psathaki
    8. Dagmar Zeuschner
    9. M Gabriele Bixel
    10. Dong Han
    11. Gabriela Morosan-Puopolo
    12. Daniela Gerovska
    13. Ji Hun Yang
    14. Jeong Beom Kim
    15. Marcos J Arauzo-Bravo
    16. Jens C Schwamborn
    17. Stephan A Hahn
    18. Ralf H Adams
    19. Hans R Schöler
    20. Matthias Vorgerd
    21. Beate Brand-Saberi
    22. Holm Zaehres
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors develop a cell culture system for studies of muscle tissue development and homeostasis. They convincingly validate a novel 3D cell model. Their thorough molecular and functional characterization will make this useful for future workers in the field.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  13. Automated cell annotation in multi-cell images using an improved CRF_ID algorithm

    This article has 10 authors:
    1. Hyun Jee Lee
    2. Jingting Liang
    3. Shivesh Chaudhary
    4. Sihoon Moon
    5. Zikai Yu
    6. Taihong Wu
    7. He Liu
    8. Myung-Kyu Choi
    9. Yun Zhang
    10. Hang Lu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This research advance article describes a valuable image analysis method to identify individual cells (neurons) within a ‎population of fluorescently labeled cells in the nematode C. elegans. The findings are solid and the method succeeds to identify cells with high precision. The method will be valuable to the C. elegans research community.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  14. Development and biophysical characterization of a humanized FSH–blocking monoclonal antibody therapeutic formulated at an ultra-high concentration

    This article has 16 authors:
    1. Satish Rojekar
    2. Anusha R Pallapati
    3. Judit Gimenez-Roig
    4. Funda Korkmaz
    5. Farhath Sultana
    6. Damini Sant
    7. Clement M Haeck
    8. Anne Macdonald
    9. Se-Min Kim
    10. Clifford J Rosen
    11. Orly Barak
    12. Marcia Meseck
    13. John Caminis
    14. Daria Lizneva
    15. Tony Yuen
    16. Mone Zaidi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This development of a highly concentrated and potentially clinically valuable antibody formulation for MS-Hu6, a first-in-class FSH-blocking humanized antibody is of potential translational importance in the management of osteoporosis, obesity, and Alzheimer's disease. The meticulous methodology is thorough and compelling in its range of techniques examining the stability and physiochemical properties of the formulated MS-Hu6.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  15. A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell derived cardiomyocytes

    This article has 11 authors:
    1. Magali Seguret
    2. Patricia Davidson
    3. Stijn Robben
    4. Charlène Jouve
    5. CĂ©line Pereira
    6. Quitterie Lelong
    7. Lucille Deshayes
    8. Cyril Cerveau
    9. Maël Le Berre
    10. Rita S. Rodrigues Ribeiro
    11. Jean-SĂ©bastien Hulot
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The manuscript provides valuable findings that will have practical implications for high throughput cultivation of cardiac tissues. The results demonstrate convincing evidence regarding the ability to create functional cardiac tissues and maintain them over periods of days to weeks. Improving the success rate of tissue integrity over longer periods of time, reproducing the physiological isoproterenol response and studying the impulse propagation in the tissues would garner a further broad interest for the platform.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  16. IL-6 signaling exacerbates hallmarks of chronic tendon disease by stimulating progenitor proliferation & migration to damage

    This article has 6 authors:
    1. Tino Stauber
    2. Greta Moschini
    3. Amro A. Hussien
    4. Patrick K. Jaeger
    5. Katrien De Bock
    6. Jess G. Snedeker
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study details an enrichment of the IL-6 signaling pathway in human tendinopathy, and applies transcriptional profiling to an advanced in vitro model to test IL-6 specific phenotypes in tendinopathy. Overall, the strength of evidence is solid yet incomplete, as transcriptomic measurements provide clarity, though functional studies including analysis of proliferation are needed to confirm these findings. This work will be of interest to stem cell biologists and immunologists.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  17. Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins

    This article has 10 authors:
    1. Dmitry Ter-Ovanesyan
    2. Tal Gilboa
    3. Bogdan Budnik
    4. Adele Nikitina
    5. Sara Whiteman
    6. Roey Lazarovits
    7. Wendy Trieu
    8. David Kalish
    9. George M Church
    10. David R Walt
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study will be of interest and valuable to scientists of many different subareas in the study of eukaryotic extracellular vesicles. The authors' efforts to improve the way we analyze EVs are highly appreciated and their results are convincing: they not only used appropriate and validated methodologies in line with current state-of-the-art but also presented alternatives to improve these approaches.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  18. Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications

    This article has 13 authors:
    1. John Se Kit Yuen Jr
    2. Michael K Saad
    3. Ning Xiang
    4. Brigid M Barrick
    5. Hailey DiCindio
    6. Chunmei Li
    7. Sabrina W Zhang
    8. Miriam Rittenberg
    9. Emily T Lew
    10. Kevin Lin Zhang
    11. Glenn Leung
    12. Jaymie A Pietropinto
    13. David L Kaplan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript describes an approach to creating fat tissue in culture for food applications. Specifically, the efforts of growing cultivated meat focus mostly on growing skeletal muscle. However, the taste component of such artificial meat would be determined by fat content. There is a significant desire and motivation to cultivate fat tissues in vitro for the purpose of the replacement of animal products. This paper provides new technological approaches to expand adipocytes and aggregate them into structures that resemble fat.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  19. Cryo-EM reveals an unprecedented binding site for NaV1.7 inhibitors enabling rational design of potent hybrid inhibitors

    This article has 17 authors:
    1. Marc Kschonsak
    2. Christine C Jao
    3. Christopher P Arthur
    4. Alexis L Rohou
    5. Philippe Bergeron
    6. Daniel F Ortwine
    7. Steven J McKerrall
    8. David H Hackos
    9. Lunbin Deng
    10. Jun Chen
    11. Tianbo Li
    12. Peter S Dragovich
    13. Matthew Volgraf
    14. Matthew R Wright
    15. Jian Payandeh
    16. Claudio Ciferri
    17. John C Tellis
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental study describes the structure-based design of novel hybrid inhibitors targeting a human sodium channel which is a pain target. Exceptionally strong evidence for key claims was produced with a structural biological pipeline for iterative structural determination of drugs complexed with an engineered sodium channel. This work is expected to be of interest to biophysicists, drug developers, neurobiologist, and pain researchers.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  20. Optimization of energy and time predicts dynamic speeds for human walking

    This article has 2 authors:
    1. Rebecca Elizabeth Carlisle
    2. Arthur D Kuo
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study presents a new optimal control cost framework to predict features of walking bouts, adding a cost function term proportional to the duration of the walking bout in addition to the conventional energetic term. While predicted optimal trajectories from simulations qualitatively matched walking data from human subjects, the evidence supporting these claims is incomplete, as some methodological choices raise questions about the strength of the authors' claims.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity