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  1. Saccharomyces cerevisiae Rev7 regulates DSB repair pathway choice through binding and blocking Mre11 nuclease and Rad50 ATPase activities

    This article has 4 authors:
    1. Sugith Badugu
    2. Kshitiza M. Dhyani
    3. Manoj Thakur
    4. Kalappa Muniyappa
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript reports important data on the interaction of Rev7 with the Rad50-Mre11-Xrs2 complex in budding yeast providing evidence that a 42 amino acid region of Rev7 is necessary and sufficient for interaction. Rev7 is found to inhibit the Rad50 ATPase and the Mre11 nuclease activities, with the exception of the ssDNA exonuclease activity. Overall, the study is incomplete: controls are lacking, there is little evidence to support the conclusion about DSB repair pathway usage, and the work on the role of Mre11 in G4 metabolism is underdeveloped.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. Regulated mRNA recruitment in dinoflagellates is reflected in hyper-variable mRNA spliced leaders and novel eIF4Es

    This article has 7 authors:
    1. Grant D. Jones
    2. Ernest P. Williams
    3. Saddef Haq
    4. Tsvetan R. Bachvaroff
    5. M. Basanta Sanchez
    6. Allen R. Place
    7. Rosemary Jagus
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study provides previously unappreciated insights into the functions of protist eIF4E 5'mRNA cap-binding protein family members, thereby contributing to a better understanding of translation regulation in these organisms. The authors provide solid evidence to support the major conclusions of the article. However, the study may further benefit from establishing whether all of the eIF4E family members are indeed involved in translation and more direct evidence for the selectivity of their binding.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. N-Terminus of Drosophila Melanogaster MSL1 Is Critical for Dosage Compensation

    This article has 6 authors:
    1. Valentin Babosha
    2. Natalia Klimenko
    3. Anastasia Revel-Muroz
    4. Evgeniya Tikhonova
    5. Pavel Georgiev
    6. Oksana Maksimenko
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this paper, the male sex-lethal (MSL) complex of proteins and RNA is studied through a domain analysis of one of its components, MSL1, and its interaction with others. While these results could be useful to researchers in the field, several studies have shown that the view that the MSL complex mediates dosage compensation is no longer considered tenable. Since there are many ways to alter viability, claims based on sex-specific viability as a reflection of dosage compensation should be viewed with much caution, and the evidence is currently considered inadequate to support the claims.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  4. Artificially inserted G-quadruplex DNA secondary structures induce long-distance chromatin activation

    This article has 7 authors:
    1. Shuvra Shekhar Roy
    2. Sulochana Bagri
    3. Avik Sengupta
    4. Claudia Regina Then
    5. Rahul Kumar
    6. Sriram Sridharan
    7. Shantanu Chowdhury
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study is valuable in that it aims to demonstrate that genomic insertion of a G4-containing sequence can be sufficient to induce chromosome loops and alter gene expression. However, the evidence remains incomplete because it has not been shown yet that the inserted sequence indeed forms G4 structures in cells and that the observed effects are caused by these structures and not by other elements present in the inserted sequence. Testing of different G4 sequences and other loci would be required to generalize the findings.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  5. N-cadherin-Presented Slit Repulsive-Cues Direct Collective Schwann cell Migration

    This article has 9 authors:
    1. Julian J.A Hoving
    2. Elizabeth Harford-Wright
    3. Patrick Wingfield-Digby
    4. Anne-Laure Cattin
    5. Mariana Campana
    6. Toby Morgan
    7. Victor Quereda
    8. Erica Torchiaro
    9. Alison C. Lloyd
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents an interesting set of findings that connects N-cadherin and glypican-4 to Slit signaling during the regulation of contact inhibition of locomotion of Schwann cells in culture. Solid evidence is provided showing that N-cadherin not only regulates cell recognition but also proper trafficking of Slit to the cell surface. An ex-vivo model demonstrates the importance of Slit signaling during cell migration but the molecular details of how N-cadherin traffics Slit to the surface and role of glypican are unclear. The data would have been strengthened with a similar interrogation of N-cadherin in this system. The work will be of interest to cell biologists studying the mechanisms behind peripheral nervous system regeneration.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. Malaria parasite resistance to azithromycin is not readily transmitted by mosquitoes

    This article has 7 authors:
    1. Hayley D. Buchanan
    2. Robyn McConville
    3. Lee M. Yeoh
    4. Michael F. Duffy
    5. Justin A. Boddey
    6. Geoffrey I. McFadden
    7. Christopher D. Goodman
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work addressed the identifcation of antimalarial drug resistance mutations that do not readily transmit to new human hosts, focusing on azithromycin resistance. The technically challenging analyses of azithromycin-resistant parasites as they traverse the mosquito host and human liver are conducted using state-of-the-art tools, including humanized mice. While the claim regarding the lack of transmission by atovaquone-resistant P. berghei is convincing, the evidence for the lack of transmission by atovaquone-resistant P. falciparum is insufficient. This work will appeal to biologists and biomedical scientists in parasitology and drug discovery, offering insights into combating antimalarial drug resistance and understanding the fitness costs associated with drug-resistant parasites.

    Reviewed by eLife, Rapid Reviews Infectious Diseases

    This article has 8 evaluationsAppears in 4 listsLatest version Latest activity
  7. Human induced pluripotent stem cell-derived cardiomyocytes to study inflammation-induced diastolic dysfunction

    This article has 11 authors:
    1. Yuki Tatekoshi
    2. Chunlei Chen
    3. Jason Shapiro
    4. Hsiang-Chun Chang
    5. Malorie Blancard
    6. Paul W. Burridge
    7. Paul Burridge
    8. Matthew Feinstein
    9. Richard D’Aquila
    10. Priscilla Hsue
    11. Hossein Ardehali
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Tatekoshi et al. endeavored to utilize human-induced pluripotent stem cell-derived cardiomyocytes to create an in vitro model of Heart Failure with preserved Ejection Fraction (HFpEF) to investigate the impacts of inflammation, sera from HIV patients, and the application of cardioprotective and antiviral medications on cardiomyocyte relaxation as a proxy for diastolic function. Their investigations revealed that inflammatory cytokines lead to an increased decay in calcium transients, a process that could be alleviated through the use of SGLT2 inhibitors and mitochondrial antioxidants. These results indicate that inflammation might contribute to diastolic dysfunction and suggest that SGLT2 inhibitors and mitochondrial antioxidants could offer cardioprotective benefits by reducing inflammation in cardiomyocytes. These valuable, yet incomplete results, can be complemented by employing biomechanical and molecular analyses, alongside validation of the therapeutic duration and dosages.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  8. Endocrine disruptor-induced epimutagenesis in vitro: Insight into molecular mechanisms

    This article has 5 authors:
    1. Jake D. Lehle
    2. Yu-Huey Lin
    3. Amanda Gomez
    4. Laura Chavez
    5. John R. McCarrey
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study is solid and reports interesting findings on the sensitivity of different cell types to EDCs in vitro. The data are valuable but the manuscript requires more experimental details. Bioinformatic analyses need to be improved and the interpretation of results and conclusions adjusted to the results.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  9. Deletion of sulfate transporter SUL1 extends yeast replicative lifespan via reduced PKA signaling instead of decreased sulfate uptake

    This article has 7 authors:
    1. Juan Long
    2. Meng Ma
    3. Yuting Chen
    4. Bo Gong
    5. Yi Zheng
    6. Hao Li
    7. Jing Yang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study by Long et al. presents valuable findings on the role of the SUL1 gene in yeast longevity, proposing that lifespan extension can occur through signaling pathways independent of its sulfate transport function, offering new insights into aging mechanisms with potential implications beyond yeast biology. However, the evidence supporting the uncoupling of SUL1's transport and signaling functions is inadequate, relying on limited lifespan analysis without measurements for nutrients and nutrient signaling status. This research is of particular interest to the aging research community, although additional experiments are needed to fully substantiate the claims.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  10. Perturbations in eIF3 subunit stoichiometry alter expression of ribosomal proteins and key components of the MAPK signaling pathway

    This article has 11 authors:
    1. Anna Herrmannová
    2. Jan JelĂ­nek
    3. Klára Pospíšilová
    4. Farkas Kerényi
    5. Tomáš Vomastek
    6. Kathleen Watt
    7. Jan Brábek
    8. Mahabub Pasha Mohammad
    9. Susan Wagner
    10. Ivan Topisirovic
    11. Leoš Shivaya Valášek
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study demonstrates mRNA-specific regulation of translation by subunits of the eukaryotic initiation factor complex 3 (eIF3) using convincing methods, data, and analyses. The investigations have generated important information that will be of interest to biologists studying translation regulation. However, the physiological significance of the gene expression changes that were observed is not clear.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  11. Tead1 reciprocally regulates adult β-cell proliferation and function to maintain glucose homeostasis

    This article has 20 authors:
    1. Jeongkyung Lee
    2. Ruya Liu
    3. Byung S. Kim
    4. Yiqun Zhang
    5. Feng Li
    6. Rajaganapti Jagannathan
    7. Ping Yang
    8. Vinny Negi
    9. Joseph Danvers
    10. Eliana Melissa Perez-Garcia
    11. Pradip K. Saha
    12. Omaima Sabek
    13. Chad J. Creighton
    14. Cristian Coarfa
    15. Mark O. Huising
    16. Hung-Ping Shih
    17. Rita Bottino
    18. Ke Ma
    19. Mousumi Moulik
    20. Vijay K. Yechoor
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The Hippo signaling pathway plays a crucial role in controlling organ size, cell proliferation, and apoptosis, though its role in endocrine pancreas development has remained unclear. In this useful work, the authors study the function of the Tead1 transcription factor, a Hippo effector, specifically in pancreatic beta cells. They provide solid evidence, using multiple different conditional knockout models to reveal Tead1's regulatory functions in insulin secretion and beta cell proliferation. However, deeper exploration of their data and incorporating findings from existing literature on this topic would provide a clearer understanding of Tead1's role in β-cell function, within or beyond the Hippo pathway.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  12. PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesis

    This article has 10 authors:
    1. Gang Liu
    2. Yunxuan Hou
    3. Xin Jin
    4. Yixue Zhang
    5. Chaoyue Sun
    6. Chengquan Huang
    7. Yujie Ren
    8. Jianmin Gao
    9. Xiuli Wang
    10. Xiumei Jiang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental work significantly advances our understanding of how FOG1 nuclear localization is regulated during erythropoiesis and megakaryopoiesis, including the role of EPO and MPL/TPO signaling in this process. The authors provide compelling evidence using both K562 and CD34+ cells that heat shock cognate B (HSCB) can promote the proteasomal degradation of TACC3 to regulate the nuclear localization of FOG1, and that this function is independent of its role in iron-sulfur cluster (ISC) biogenesis. The conclusions would be strengthened in the future by the use of in vivo model systems, however, as written, this work will be of broad interest to cell biologists.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  13. Sperm motility in mice with Oligo-astheno-teratozoospermia restored by in vivo injection and electroporation of naked mRNA

    This article has 22 authors:
    1. Charline Vilpreux
    2. Guillaume Martinez
    3. Magali Court
    4. Florence Appaix
    5. Jean-Luc Duteyrat
    6. Maxime Henry
    7. Julien Vollaire
    8. Camille Ayad
    9. Altan Yavz
    10. Lisa De Macedo
    11. Geneviève Chevalier
    12. Emeline Lambert
    13. Sekou Ahmed Conte
    14. Elsa Giordani
    15. VĂ©ronique Josserand
    16. Jacques Brocard
    17. Coutton Charles
    18. Bernard Verrier
    19. Pierre F. Ray
    20. Corinne Loeuillet
    21. Christophe Arnoult
    22. Jessica Escoffier
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This potentially useful study reports a new method for restoring sperm motility. Strengths are in the methodology being developed, but the conclusions require additional experimental support. The authors provide inadequate evidence for the success of the method or its mechanism.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  14. A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein

    This article has 8 authors:
    1. Markéta Častorálová
    2. Jakub Sýs
    3. Jan Prchal
    4. Anna PavlĹŻ
    5. Lucie Prokopová
    6. Zina Briki
    7. Martin Hubálek
    8. Tomas Ruml
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study advances our understanding of how the viral protease in a D-type retrovirus is activated and in particular how the exposure of the myristoyl group is required for processing of the Gag matrix precursor. The supporting evidence is convincing, but the work would benefit from additional data in support of the claims. This manuscript is of interest to retrovirologists and structural biologists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  15. Aberrant methylation and expression of TNXB promotes chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling

    This article has 12 authors:
    1. Jiali Chen
    2. Zeng Qinghe
    3. Xu Wang
    4. Rui Xu
    5. Weidong Wang
    6. Yuliang Huang
    7. Qi Sun
    8. Wenhua Yuan
    9. Pinger Wang
    10. Di Chen
    11. Peijian Tong
    12. Hongting Jin
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study identifies the TNXB-AKT pathway as a potential mechanism underlying hemophilia-associated cartilage degeneration. The evidence supporting the conclusions is convincing, with murine and human patient evidence as well as genome-wide DNA methylation analysis. This paper would be of interest to cell biologists and biochemists working on musculoskeletal disorders.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  16. Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9

    This article has 5 authors:
    1. Joshua D Eaton
    2. Jessica Board
    3. Lee Davidson
    4. Chris Estell
    5. Steven West
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The important study uses a new experimental method to provide compelling evidence on how sense- and anti-sense transcription is differentially regulated. The method described here can generally be used to study the alterations in transcription. This paper will be of interest to scientists working in the gene regulation community.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  17. Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progression

    This article has 6 authors:
    1. Zulong Chen
    2. Xin Wang
    3. Xinlei Gao
    4. Nina Arslanovic
    5. Kaifu Chen
    6. Jessica Tyler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work describes a compelling analysis of DNA damage-induced changes in nascent RNA transcripts, and a genome-wide screening effort to identify the responsible proteins. A significant discovery is the inability of arrested cells to undergo DNA damage-induced gene silencing, which, is attributed to an inability to mediate ATM-induced transcriptional repression. Revisions are suggested that would significantly enhance and support the central claims of the study. This work will be of general interest to the DNA damage, repair, and transcription fields, with a potential impact on the cancer field.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  18. The Molecular Logic of Gtr1/2 and Pib2 Dependent TORC1 Regulation in Budding Yeast

    This article has 6 authors:
    1. Jacob H. Cecil
    2. Cristina M. Padilla
    3. Austin A. Lipinski
    4. Paul R. Langlais
    5. Xiangxia Luo
    6. Andrew P. Capaldi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study presents valuable findings concerning how a highly conserved signal transduction pathway helps budding yeast cells adapt their growth to nitrogen sources of differing qualities. However, the evidence is incomplete for the authors' main claim that the pathway adopts three distinct states depending on the nitrogen source. The presented data, particularly phospho-proteomic datasets, will be of interest to the cell growth signaling community.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  19. Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions

    This article has 5 authors:
    1. Ting-Wen Chen
    2. Hsiao-Wei Liao
    3. Michelle Noble
    4. Jing-Yi Siao
    5. Chung-Te Chang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This useful study provides incomplete evidence for the functional roles of the human DCP1 paralogs in regulating RNA decay by DCP2. Using a combination of cellular-based assays and in vitro assays, the authors conclude that DCP1a/b plays a role in regulating DCP2 activity. This study makes a number of interesting and potentially relevant observations; however, a number of outstanding questions remain to be addressed. These results will be of interest to the RNA community.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 2 listsLatest version Latest activity
  20. PURA Syndrome-causing mutations impair PUR-domain integrity and affect P-body association

    This article has 17 authors:
    1. Marcel Proske
    2. Robert Janowski
    3. Sabrina Bacher
    4. Hyun-Seo Kang
    5. Thomas Monecke
    6. Tony Köhler
    7. Saskia Hutten
    8. Jana Tretter
    9. Anna Crois
    10. Lena Molitor
    11. Alejandro Varela-Rial
    12. Roberto Fino
    13. Elisa Donati
    14. Gianni De Fabritiis
    15. Dorothee Dormann
    16. Michael Sattler
    17. Dierk Niessing
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study addresses the mechanisms by which mutations in the PURA protein, a regulator of gene transcription and mRNA transport and translation, cause the neurodevelopmental PURA syndrome. Based on convincing evidence from structural biology, molecular dynamics simulation, biochemical, and cell biological analyses, the authors show that the PURA structure is very dynamic, rendering it generally sensitive to structure-altering mutations that affect its folding, DNA-unwinding activity, RNA binding, dimerization, and partitioning into processing bodies. These findings are of substantial importance to cell biology, neurogenetics, and neurology alike, because they provide first insights into how very diverse PURA mutations can cause similar and penetrant molecular, cellular, and clinical defects.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity