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  1. Impaired Myofibroblast Proliferation is a Central Feature of Pathologic Post-Natal Alveolar Simplification

    This article has 8 authors:
    1. Imran S. Khan
    2. Christopher Molina
    3. Xin Ren
    4. Vincent C. Auyeung
    5. Max Cohen
    6. Tatsuya Tsukui
    7. Amha Atakilit
    8. Dean Sheppard
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study unveils important mechanistic insights into postnatal lung development and bronchopulmonary dysplasia (BPD) pathology. Using two BPD models enhances our comprehension of the disease, utilizing compelling evidence from single-cell sequencing and flow cytometry, revealing a myofibroblast loss. Pharmacological and genetic approaches convincingly argue against the presumed increase in TGFb signaling causing alveolar simplification; instead, it appears to be a compensatory response. The identified weakness is the absence of validation in tissue, leaving the question unanswered regarding whether myofibroblast loss is due to a lack of myofibroblast proliferation or myofibroblast differentiation/specification.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. Remodelling of skeletal muscle myosin metabolic states in hibernating mammals

    This article has 23 authors:
    1. Christopher T. A. Lewis
    2. Elise G. Melhedegaard
    3. Marija M. Ognjanovic
    4. Mathilde S. Olsen
    5. Jenni Laitila
    6. Robert A. E. Seaborne
    7. Magnus Nørregaard Grønset
    8. Chengxin Zhang
    9. Hiroyuki Iwamoto
    10. Anthony L. Hessel
    11. Michel N. Kuehn
    12. Carla Merino
    13. Nuria Amigó
    14. Ole Fröbert
    15. Sylvain Giroud
    16. James F. Staples
    17. Anna V. Goropashnaya
    18. Vadim B. Fedorov
    19. Brian M. Barnes
    20. Øivind Tøien
    21. Kelly L. Drew
    22. Ryan J. Sprenger
    23. Julien Ochala
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The work by Lewis and co-workers presents important findings on the role of myosin structure/energetics on the molecular mechanisms of hibernation by comparing muscle samples from small and large hibernating mammals. The solid methodological approaches have revealed insights into the mechanisms of non-shivering thermogenesis and energy expenditure.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. Reliance on self-reports and estimated food composition data in nutrition research introduces significant bias that can only be addressed with biomarkers

    This article has 4 authors:
    1. Javier I. Ottaviani
    2. Virag Sagi-Kiss
    3. Hagen Schroeter
    4. Gunter G. C. Kuhnle
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study, using three bioactive compounds as a model, demonstrates that estimating the intake of food components based on food composition databases and self-reported dietary data is highly unreliable. The authors present convincing data showing the differences in the estimated quantile of intake of three bioactive compounds between biomarker and 24-hour dietary recall with food-composition database. The work will be of broad interest to the clinical nutrition research community.

    Reviewed by eLife

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  4. Loss of CTRP10 results in female obesity with preserved metabolic health

    This article has 7 authors:
    1. Fangluo Chen
    2. Dylan C. Sarver
    3. Muzna Saqib
    4. Leandro M Velez
    5. Susan Aja
    6. Marcus M. Seldin
    7. G. William Wong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript presents a detailed characterization of male and female wild-type and CTRP10 knockout mice, revealing that knockout mice develop female-specific obesity that is largely uncoupled from metabolic dysfunction. The data are convincing, and the work is a valuable contribution to understanding how obesity is coupled to metabolic dysfunction, and how this can occur in a sex-specific manner.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  5. Exceptional longevity of mammalian ovarian and oocyte macromolecules throughout the reproductive lifespan

    This article has 8 authors:
    1. Ewa K. Bomba-Warczak
    2. Karen M. Velez
    3. Luhan T Zhou
    4. Christelle Guillermier
    5. Seby Edassery
    6. Matthew Steinhauser
    7. Jeffrey N. Savas
    8. Francesca E. Duncan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents important findings on long-lived proteins in the mouse ovary and oocytes. Convincing evidence using two-generation stable isotope-based metabolic labelling of mice in combination with mass spectrometry analysis describes a resource of enriched long-lived macromolecules in oocytes and somatic cells. Mitochondrial, cytoskeletal, and myosin proteins were identified as long-lived. While this is a unique resource with significant value in the understanding of female reproductive aging, the results would be strengthened if supported by an orthogonal validation and a more in-depth mechanistic explanation of the relationship between mitochondrial and cytoskeletal protein stability and aging.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. Hammerhead-type FXR agonists induce an eRNA FincoR that ameliorates nonalcoholic steatohepatitis in mice

    This article has 7 authors:
    1. Jinjing Chen
    2. Ruoyu Wang
    3. Feng Xiong
    4. Hao Sun
    5. Byron Kemper
    6. Wenbo Li
    7. Jongsook Kim Kemper
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment:

      The authors report a novel hepatic lncRNA FincoR regulating FXR with therapeutic implications in the treatment of MASH. The findings are important and use an appropriate methodology in line with the current state-of-the-art, with convincing support for the claims.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  7. Involvement of TRPV4 in temperature-dependent perspiration in mice

    This article has 7 authors:
    1. Makiko Kashio
    2. Sandra Derouiche
    3. Reko U. Yoshimoto
    4. Kenji Sano
    5. Jing Lei
    6. Mizuho A. Kido
    7. Makoto Tominaga
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      TRPV4 is a unique cation channel that has been demonstrated to play a role in a variety of sensory processes. The authors provide useful new data to indicate that TRPV4 activation occurs in eccrine gland cells. They then show that temperature-dependent perspiration is TRPV4-dependent in mouse skin. This provides new insight, but the data are incomplete in that more orthogonal assays could be used to more comprehensively support the conclusions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. Maresin 1 repletion improves muscle regeneration after volumetric muscle loss

    This article has 13 authors:
    1. Jesus A Castor-Macias
    2. Jacqueline A Larouche
    3. Emily C Wallace
    4. Bonnie D Spence
    5. Alec Eames
    6. Pamela Duran
    7. Benjamin A Yang
    8. Paula M Fraczek
    9. Carol A Davis
    10. Susan V Brooks
    11. Krishna Rao Maddipati
    12. James F Markworth
    13. Carlos A Aguilar
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is a compelling study on pro-inflammatory and anti-inflammatory lipids in relation to skeletal muscle injury. It convincingly identifies pro-inflammatory lipids during recovery predisposing to fibrosis, and maresin 1 as an anti-inflammatory lipid reducing fibrosis, improved muscle regeneration, partially restoring contractile function, of fundamental potential clinical application.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  9. Deletion of FNDC5/Irisin modifies murine osteocyte function in a sex-specific manner

    This article has 9 authors:
    1. Anika Shimonty
    2. Fabrizio Pin
    3. Matt Prideaux
    4. Gang Peng
    5. Joshua R Huot
    6. Hyeonwoo Kim
    7. Clifford J Rosen
    8. Bruce M Spiegelman
    9. Lynda F Bonewald
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study presents a valuable finding on sexually-dimorphic patterns of osteocyte transcriptomics and low calcium diet-induce bone loss. The evidence supporting the claims of the authors regarding the protective role of Irisin/FNDC5-deficiency in lactation and low-calcium diet in female mice is incomplete, due to the relatively modest phenotypes observed and the insufficient link between Irisin/FNDC5 and calcium homeostasis or milk production.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 3 listsLatest version Latest activity
  10. A kidney-hypothalamus axis promotes compensatory glucose production in response to glycosuria

    This article has 9 authors:
    1. Tumininu S. Faniyan
    2. Xinyi Zhang
    3. Donald A. Morgan
    4. Jorge Robles
    5. Siresha Bathina
    6. Paul S. Brookes
    7. Kamal Rahmouni
    8. Rachel J. Perry
    9. Kavaljit H. Chhabra
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a useful characterization of mechanisms underlying glycosuria-mediated increase in compensatory glucose production in Glut2 knockout mice. The strength of support is incomplete but the data represent a starting point for further studies regarding the role of the HPA axis and acute phase proteins in regulating blood glucose during glycosuria.

    Reviewed by eLife, PREreview

    This article has 5 evaluationsAppears in 3 listsLatest version Latest activity
  11. Itaconate stabilizes CPT1a to enhance lipid utilization during inflammation

    This article has 7 authors:
    1. Rabina Mainali
    2. Nancy Buechler
    3. Cristian Otero
    4. Laken Edwards
    5. Chia-Chi Key
    6. Cristina Furdui
    7. Matthew A Quinn
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work describes a connection between inflammation and metabolism, in which itaconate stabilizes the mitochondrial fatty acid uptake enzyme Cpt1a to enhance fatty acid oxidation. The mechanism for itaconate action may be generalizable to other protein targets. This is an important advance, which is supported by solid experimental data.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  12. A 2-Hydroxybutyrate- mediated feedback loop regulates muscular fatigue

    This article has 14 authors:
    1. Brennan J Wadsworth
    2. Marina Leiwe
    3. Eleanor A Minogue
    4. Pedro P Cunha
    5. Viktor Engman
    6. Carolin Brombach
    7. Christos Asvestis
    8. Shiv K Sah-Teli
    9. Emilia Marklund
    10. Peppi Koivunen
    11. Jorge L Ruas
    12. Helene Rundqvist
    13. Johanna T Lanner
    14. Randall S Johnson
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The work by Johnson and co-workers has identified an important role of 2-Hydroxybutyrate in skeletal muscle oxidative capacity in the early stages of exercise. Mechanistically, they show convincing data to support a role of 2-Hydroxybutyrate in the regulation of BCAA metabolism via SIRT4, ADP-Ribosylation, and CEBP. However, whether this is the sole mechanism and if these translate to longer exercise training regimes requires future experiments.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  13. Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila

    This article has 10 authors:
    1. Sophie A Fleck
    2. Puja Biswas
    3. Emily D DeWitt
    4. Rebecca L Knuteson
    5. Robert C Eisman
    6. Travis Nemkov
    7. Angelo D'Alessandro
    8. Jason M Tennessen
    9. Elizabeth Rideout
    10. Lesley N Weaver
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study shows that auxin exposure perturbs feeding behavior, survival rates, lipid metabolism, and gene expression patterns in adult Drosophila flies. The results are solid with proper methods and data analyses, and the evidence broadly supports the conclusions with only minor weaknesses. This work is relevant for fly geneticists who are interested in using the auxin-inducible gene expression system for inducing target protein degradation acutely.

    Reviewed by eLife

    This article has 10 evaluationsAppears in 2 listsLatest version Latest activity
  14. Sympathetic Motor Neuron Dysfunction is a Missing Link in Age-Associated Sympathetic Overactivity

    This article has 4 authors:
    1. Lizbeth de la Cruz
    2. Derek Bui
    3. Claudia M. Moreno
    4. Oscar Vivas
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents valuable observations indicating that the electrophysiological excitability of cultured sympathetic motor neurons progressively increase during aging, and are inversely correlated with the magnitude of KCNQ currents. The alterations in membrane excitability are broadly relevant for those interested in understanding how the nervous system changes during aging. While the data as a whole are solid in showing that the excitability of sympathetic neurons increases in neurons cultured from older mice, the mechanism of the underlying changes and in vivo relevance is incomplete.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  15. Lymphatic muscle cells are the innate pacemaker cells regulating mouse lymphatic collecting vessel contractions

    This article has 10 authors:
    1. S.D. Zawieja
    2. G.A. Pea
    3. S.E. Broyhill
    4. K.H. Bromert
    5. C. E. Norton
    6. H. J. Kim
    7. M. Li
    8. J.A. Castorena-Gonzalez
    9. B.T. Drumm
    10. M.J. Davis
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript aims to identify the pacemaker cells in the lymphatic collecting vessels - the cells that initiate the autonomous action potentials and contractions needed to drive lymphatic pumping. Through the exemplary use of existing approaches (genetic deletions and cytosolic calcium detection in multiple cell types), the authors convincingly determine that lymphatic muscle cells are the origin of the action potential that triggers lymphatic contraction. This fundamental discovery establishes a new standard for the field of lymphatic physiology.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  16. Resistance exercise protects mice from protein-induced fat accretion

    This article has 11 authors:
    1. Michaela E Trautman
    2. Leah N Braucher
    3. Christian Elliehausen
    4. Wenyuan G Zhu
    5. Esther Zelenovskiy
    6. Madelyn Green
    7. Michelle M Sonsalla
    8. Chung-Yang Yeh
    9. Troy A Hornberger
    10. Adam R Konopka
    11. Dudley W Lamming
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable finding on relationship between high protein diet and resistance exercise on fat accumulation and glucose homeostasis. The evidence supporting the claims of the authors is solid, although inclusion of mechanistic insight would have strengthened the study. The work will be of interest to dietician and exercise biologists working to understand the synergy between diet and physical activity.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  17. OptoPI3K, genetic code expansion, and click chemistry reveal mechanisms underlying reciprocal regulation between TRPV1 and PI3K

    This article has 12 authors:
    1. Duk-Su Koh
    2. Anastasiia Stratiievska
    3. Subhashis Jana
    4. Shauna C. Otto
    5. Teresa M. Swanson
    6. Anthony Nhim
    7. Sara Carlson
    8. Marium Raza
    9. Lígia Araujo Naves
    10. Eric N. Senning
    11. Ryan Mehl
    12. Sharona E. Gordon
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study develops an important method for dissecting out two overlapping cell signaling pathways, phosphoinositide signaling and membrane protein trafficking. The combination of two state-of-the-art techniques provides compelling evidence for a reciprocal influence between an enzyme and a channel. The work will be of interest to the broader cell biology, biophysics and biochemistry communities.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  18. Intermittent fasting promotes ILC3s secreting IL-22 contributing to the beigeing of white adipose tissue

    This article has 10 authors:
    1. Hong Chen
    2. Lijun Sun
    3. Lu Feng
    4. Xue Han
    5. Yunhua Zhang
    6. Wenbo Zhai
    7. Zehe Zhang
    8. Michael Mulholland
    9. Weizhen Zhang
    10. Yue Yin
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides valuable findings showing the production of IL-22 from intestinal ILC3 during intermittent fasting promotes beigeing of white adipose tissue. The authors provided solid data and mechanistic insight by which IL-22-derived from ILC3 directly induces beigeing.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 2 listsLatest version Latest activity
  19. CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice

    This article has 6 authors:
    1. Jenny Clarkson
    2. Siew Hoong Yip
    3. Robert Porteous
    4. Alexia Kauff
    5. Alison K Heather
    6. Allan E Herbison
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study provides convincing evidence of the criticality of estradiol – estrogen receptor-mediated upregulation of kisspeptin within neurons of the preoptic area to generate an ovulation-inducing luteinizing hormone surge. The use of in vivo CRIPSR-Cas9 is novel in this system and provides a road map for future studies in reproductive neuroendocrinology. This paper will be of interest to reproductive neuroscientists and endocrinologists.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 2 listsLatest version Latest activity
  20. Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

    This article has 5 authors:
    1. Dylan C Sarver
    2. Jean Garcia-Diaz
    3. Muzna Saqib
    4. Ryan C Riddle
    5. G William Wong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study discloses important physiological function for TMEM63 in regulating postnatal growth in mice. The data supporting the impaired body growth and skeletal phenotype as well as disrupted growth hormone/insulin-like growth factor-I (GH/IGF-I) signaling in TMEM63 knockout mice are compelling. However, to establish that alteration of hepatic GH/IGF-I signaling is the cause for observed growth and skeletal phenotype in TMEM63 knockout mice would need additional work.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity