Showing page 1 of 4 pages of list content

  1. Improving rice drought tolerance through host-mediated microbiome selection

    This article has 4 authors:
    1. Alex Styer
    2. Dean Pettinga
    3. Daniel Caddell
    4. Devin Coleman-Derr
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study reports on a series of artificial selection experiments for microbiomes that confer drought tolerance to rice plants. A major strength is the solid experimental design with multiple soils, which will likely guide others in designing their experiments, but the study has also shortcomings in that the rescuing effect is not benchmarked against healthy well-watered plants, the sterilized controls do not add much information, and the dispersal between inocula confounds the interpretation of the results. In addition, while the type of work presented here is a first step towards the eventual goal of plant microbiome engineering, that goal is still mainly an ambition. The abstract would benefit from this being made clear, and the presentation would overall benefit from more extensive consideration of recent developments in the field.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. ATG6 interacting with NPR1 increases Arabidopsis thaliana resistance to Pst DC3000/avrRps4 by increasing its nuclear accumulation and stability

    This article has 10 authors:
    1. Baihong Zhang
    2. Shuqin Huang
    3. Shuyu Guo
    4. Yixuan Meng
    5. Yuzhen Tian
    6. Yue Zhou
    7. Hang Chen
    8. Xue Li
    9. Jun Zhou
    10. Wenli Chen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study reports on a previously unrecognized function of ATG6 in plant immunity. The work is valuable because it proposes a direct interaction between ATG6 and a well-studied salicylic acid receptor protein, NPR1, which may interest researchers investigating plant immunity regulation. While the data presented are compelling, more information regarding the specificity of ATG6's role would improve the overall impact of the study, especially with an eye towards consistency with prior work.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  3. A shade-responsive microProtein in the Arabidopsis ATHB2 gene regulates elongation growth and root development

    This article has 12 authors:
    1. Ashleigh Edwards
    2. Maurizio Junior Chiurazzi
    3. Anko Blaakmeer
    4. Ylenia Vittozzi
    5. Ashish Sharma
    6. Sanne Matton
    7. Valdeko Kruusvee
    8. Daniel Straub
    9. Giovanna Sessa
    10. Monica Carabelli
    11. Giorgio Morelli
    12. Stephan Wenkel
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Through a genome-wide screen for functional alternative transcription start sites (TSS) in Arabidopsis, the authors provide evidence for widespread transcription of potential microproteins from previously annotated protein-coding genes. Functional analysis of AtHB2-miP, derived from the C-terminal region of transcription factor AtHB2 and predicted to form non-productive dimers with ATHB2, suggested that this microprotein could affect AtHB2 functions in shade responses, root growth, and iron homeostasis. The work is valuable as a case study of how new microproteins could act to modulate gene regulation in response to environmental change, but the focus on a single gene, the lack of precision in AtHB2-miP measurement with missing controls, and the relatively minor phenotypic effects in the specific case investigated, leave it unclear how important microprotein production is as a general regulatory strategy.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  4. Pectin methylesterase activity is required for RALF1 peptide signalling output

    This article has 8 authors:
    1. Ann-Kathrin Rößling
    2. Kai DĂĽnser
    3. Chenlu Liu
    4. Susan Lauw
    5. Marta Rodriguez-Franco
    6. Lothar Kalmbach
    7. Elke Barbez
    8. JĂĽrgen Kleine-Vehn
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental study provides mostly convincing evidence for pectin modification as a requirement for RALF peptide signalling altering the apoplastic pH, adding further support for a key role of RALF peptides in linking the assembly and dynamics of the extracellular matrix with cellular activity and function. A small number of additional controls would further enhance the study.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  5. Unraveling the Role of Urea Hydrolysis in Salt Stress Response during Seed Germination and Seedling Growth in Arabidopsis thaliana

    This article has 8 authors:
    1. Yuanyuan Bu
    2. Xingye Dong
    3. Rongrong Zhang
    4. Xianglian Shen
    5. Yan Liu
    6. Shu Wang
    7. Tetsuo Takano
    8. Shenkui Liu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study advances our understanding of the molecular mechanism underlying salt stress-induced inhibition of seed germination and seedling growth. The evidence supporting the conclusions is convincing, with rigorous genetic, physiological, and metabolic analyses. This paper will be of interest to plant stress biologists and crop breeders.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. In vitro function, assembly, and interaction of primary cell wall cellulose synthase homotrimers

    This article has 3 authors:
    1. Pallinti Purushotham
    2. Ruoya Ho
    3. Jochen Zimmer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      It is well-established that cellulose synthesis in higher plants requires three different but related catalytic subunits known as CESA proteins. Here the authors provide convincing cryo-electron microscopy structural information on soybean CESA1, CESA3, and CESA6 and find substantial differences between the structure of these CESA homotrimers and the previously-resolved secondary cell wall CESAs. They present an important model in which the multi-subunit cellulose synthase complexes are made of multiple homotrimers but further evidence is needed to strengthen the conclusions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  7. Root cap cell corpse clearance limits microbial colonization in Arabidopsis thaliana

    This article has 6 authors:
    1. Nyasha M. Charura
    2. Ernesto Llamas
    3. Concetta De Quattro
    4. David Vilchez
    5. Moritz K. Nowack
    6. Alga Zuccaro
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study explores the interplay between fungal colonization and controlled programmed cell death in Arabidopsis thaliana root cells. The authors reveal how this process is affected by corpse clearance in the root cap, highlighting some of the key elements in this process including a root cap-specific transcription factor. With this, the authors have discovered an important relationship between transcriptional regulation of developmentally controlled cell death and the beneficial colonization of plants by fungi. The work thus establishes a solid basis for further studies of plant-microbiome associations along the root axis.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  8. OsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo

    This article has 5 authors:
    1. Zongju Yang
    2. Tianqi Bai
    3. E Zhiguo
    4. Baixiao Niu
    5. Chen Chen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is an important study on the regulation of chlorophyll biosynthesis in rice embryos. It provides insights into the genetic and molecular interactions that underlie chlorophyll accumulation, highlighting the inhibition of OsGLK1 by OsNF-YB7 and the broader implications for understanding chloroplast development and seed maturation in angiosperms. The results presented, including mutation analysis, gene expression profiles, and protein interaction studies, provide convincing evidence for the function of OsNF-YB7 as a repressor in the chlorophyll biosynthesis pathway.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  9. Distinct effects of phyllosphere and rhizosphere microbes on invader Ageratina adenophora during its early life stages

    This article has 7 authors:
    1. Zhao-Ying Zeng
    2. Jun-Rong Huang
    3. Zi-Qing Liu
    4. Ai-Ling Yang
    5. Yu-Xuan Li
    6. Yong-Lan Wang
    7. Han-Bo Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study advances our understanding of how leaf and soil microbes separately affect the performance of an invasive plant, Ageratina adenophora. The conclusions regarding the roles of litter microbes in regulating the A. adenophora population are currently supported only by incomplete evidence owing to limitations in experimental design and statistical analyses, as well as uncertainties associated with the presentation. The work will be of interest to invasion biologists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  10. Serine hydroxymethyl transferase is a binding target of caprylic acid: Uncovering a novel molecular target for a herbicide and for producing caprylic acid-tolerant crops

    This article has 11 authors:
    1. Zuren Li
    2. Mugui Wang
    3. Haodong Bai
    4. Hongzhi Wang
    5. Jincai Han
    6. Likun An
    7. Dingfeng Luo
    8. Yingying Wang
    9. Wei Kuang
    10. Xiaoyi Nie
    11. Lianyang Bai
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable contribution towards understanding the protein target and mechanism of action of an herbicide, which could be applied to the development of herbicide-based technologies to improve crop yields. Evidence is gathered using a variety of technical approaches that enrich and support the findings, but the methodology and the presentation of the results are incomplete.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  11. Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation

    This article has 6 authors:
    1. Xinyu Chen
    2. Huasong Zou
    3. Tao Zhuo
    4. Wei Rou
    5. Wei Wu
    6. Xiaojing Fan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study provides new insight into potential subtle dynamics in effector biology. The data presented generally support the claims, but in some cases controls are missing and so the overall work is currently incomplete. If the limitations can be addressed, this work should be of broad relevance for biologists interested in molecular plant-microbe interactions.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  12. Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts

    This article has 7 authors:
    1. Masanori Izumi
    2. Sakuya Nakamura
    3. Kohei Otomo
    4. Hiroyuki Ishida
    5. Jun Hidema
    6. Tomomi Nemoto
    7. Shinya Hagihara
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript investigates how chloroplasts are broken down during light-limiting conditions as plants reorganize their energy-producing organelles during carbon limitation. The authors provide convincing live-cell imaging data of plastids, documenting that buds form on the surface of chloroplasts and pinch away, then associate with the vacuole via a mechanism that depends on autophagy machinery, but not plastid division machinery. However, the absence of quantitative analyses makes the work incomplete at the current stage. The manuscript nevertheless provides important groundwork for other scientists studying the regulation and breakdown of energy-producing organelles, including chloroplasts and mitochondria.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  13. Soybean RIN4 represents a mechanistic link between plant immune and symbiotic signaling

    This article has 10 authors:
    1. Katalin TĂłth
    2. Daewon Kim
    3. Sung-Hwan Cho
    4. Cuong T. Nguyen
    5. Tran H. N. Nguyen
    6. Christopher Hartanto
    7. Jean-Michel Michno
    8. Adrian O. Stec
    9. Robert M. Stupar
    10. Gary Stacey
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The manuscript describes important findings supported by convincing data. The authors present persuasive genetic and biochemical evidence that supports the biological mechanism for optimal nodulation in soybean presented in this study. The results are of relevance to understanding the signaling pathway underpinning beneficial rhizobia symbiosis, while repressing the immune response. With the discussion part strengthened this paper would be of broad interest to plant biologists working on cell signaling and plant-microbe interactions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  14. Natural genetic variation underlying the negative effect of elevated CO2 on ionome composition in Arabidopsis thaliana

    This article has 10 authors:
    1. Océane Cassan
    2. Léa-Lou Pimparé
    3. Timothy Mozzanino
    4. CĂ©cile Fizames
    5. SĂ©bastien Devidal
    6. Fabrice Roux
    7. Alexandru Milcu
    8. Sophie Lèbre
    9. Alain Gojon
    10. Antoine Martin
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper provides useful information about how the ionome of Arabidopsis thaliana adapts to very high CO2-levels, backed up by solid evidence and carefully designed studies. The work will be of interest to anyone studying natural genetic variation as well as the response of plants to altered CO2 levels in the atmosphere.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  15. A translation proofreader of archaeal origin imparts multi-aldehyde stress tolerance to land plants

    This article has 12 authors:
    1. Pradeep Kumar
    2. Ankit Roy
    3. Shivapura Jagadeesha Mukul
    4. Avinash Kumar Singh
    5. Dipesh Kumar Singh
    6. Aswan Nalli
    7. Pujaita Banerjee
    8. Kandhalu Sagadevan Dinesh Babu
    9. Bakthisaran Raman
    10. Shobha P Kruparani
    11. Imran Siddiqi
    12. Rajan Sankaranarayanan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The work is a fundamental contribution towards understanding the role of archaeal and plant D-aminoacyl-tRNA deacylase 2 (DTD2) in deacylation and detoxification of D-Tyr-tRNATyr modified by various aldehydes produced as metabolic byproducts in plants. It integrates convincing results from both in vitro and in vivo experiments to address the long-standing puzzle of why plants outperform bacteria in handling reactive aldehydes and suggests a new strategy for stress-tolerant crops. A limitation of the study is the lack of evidence for accumulation of toxic D-aminoacyl tRNAs and impairment of translation in plant cells lacking DTD2.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  16. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

    This article has 5 authors:
    1. Ivan Kulich
    2. Julia Schmid
    3. Anastasia Teplova
    4. Linlin Qi
    5. Jiří Friml
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental study addresses the earliest events that enable plant roots to reorient growth in response to gravity. Compelling molecular and cell biological data establish that plasma membrane localization of the LAZY or NEGATIVE GRAVITROPIC RESPONSE OF ROOTS (NGR) protein family is required for rapid and polar redirection of D6 protein kinase, an activator of the PIN3 auxin transporter. This work complements and extends recent publications on the NGR family in gravity sensing (PMID: 37741279 and PMID: 37561884). Collectively these papers advance our understanding of rapid plant gravity sensing and response.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  17. Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

    This article has 7 authors:
    1. Lucie Crhak Khaitova
    2. Pavlina Mikulkova
    3. Jana Pecinkova
    4. Manikandan Kalidass
    5. Stefan Heckmann
    6. Inna Lermontova
    7. Karel Riha
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study is an important contribution to our insights into the impact of heat stress on sexual reproduction in plants and provides information about how centromere integrity is affected by heat stress during male meiosis in Arabidopsis thaliana. The evidence supporting the claims, specifically the dynamics of tagged proteins in meiocytes by live cell imaging is solid, even though a deeper mechanistic understanding is still lacking.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  18. H1 restricts euchromatin-associated methylation pathways from heterochromatic encroachment

    This article has 5 authors:
    1. C. Jake Harris
    2. Zhenhui Zhong
    3. Lucia Ichino
    4. Suhua Feng
    5. Steven E. Jacobsen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study indicates a role for linker Histone H1 in protecting heterochromatic regions from certain types of repression. The experiments and data analysis that support the model for the role of linker Histone H1are solid, although additional experiments could provide a deeper mechanistic understanding. The study will be of broad interest to those interested in the role of chromatin in eukaryotic gene expression.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  19. Interdependence of a kinase and its cognate substrate plasma membrane nanoscale dynamics underlies Arabidopsis response to viral infection

    This article has 22 authors:
    1. Marie-Dominique Jolivet
    2. Anne-Flore Deroubaix
    3. Marie Boudsocq
    4. Nikolaj B. Abel
    5. Marion Rocher
    6. Terezinha Robbe
    7. Valérie Wattelet-Boyer
    8. Jennifer Huard
    9. Dorian Lefebvre
    10. Yi-Ju Lu
    11. Brad Day
    12. Grégoire Saias
    13. Jahed Ahmed
    14. Valérie Cotelle
    15. Nathalie Giovinazzo
    16. Jean-Luc Gallois
    17. Yasuyuki Yamaji
    18. Sylvie German-Retana
    19. Julien Gronnier
    20. Thomas Ott
    21. SĂ©bastien Mongrand
    22. VĂ©ronique Germain
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study describes how the composition and stabilization of nanodomains in the plasma membrane are an integral part of plant defence against viruses, with a focus on the calcium-dependent kinase CPK3 and its apparent interaction with a plasma-membrane nano domain scaffold protein from the remorin family. While the evidence for a specific role of CPK3 in limiting viral spread is convincing, the claims regarding the CPK3-remorin interaction would be strengthened by additional experimental support. The work, which will be of interest to plant cell biologists and plant virology, opens new avenues for understanding the role of plasma membrane nanodomains in limiting viral spread.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  20. Vicia faba SV channel VfTPC1 is a hyperexcitable variant of plant vacuole Two Pore Channels

    This article has 12 authors:
    1. Jinping Lu
    2. Ingo Dreyer
    3. Miles Sasha Dickinson
    4. Sabine Panzer
    5. Dawid Jaślan
    6. Carlos Navarro-Retamal
    7. Dietmar Geiger
    8. Ulrich Terpitz
    9. Dirk Becker
    10. Robert M Stroud
    11. Irene Marten
    12. Rainer Hedrich
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Plant intracellular ion channels are poorly understood. In this important manuscript, patch-clamp is used to define functional differences between two cation channels present in the vacuole of different plants. The authors find a calcium-biding site whose absence or presence modulate activation at lower voltages and is responsible for increased excitability in the vacuole of the faba bean plant. The experimental evidence presented is convincing and findings have practical implications for the field of plant electrophysiology and channel biophysics.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity