Differential Expression of 5’-tRNA Fragments in Circulating Preeclampsia Syncytiotrophoblast Vesicles Drives Macrophage Inflammation
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Background
The relationship between placental pathology and the maternal syndrome of preeclampsia is incompletely characterised. Mismatch between placental nutrient supply and fetal demands induces stress in the syncytiotrophoblast, the layer of placenta in direct contact with maternal blood. Such stress alters the content and increases the release of extracellular vesicles (STB-EVs) into the maternal circulation. We have previously shown 5’-tRNA fragments (5’-tRFs) constitute the majority of small RNA in STB-EVs in healthy pregnancy. 5’-tRFs are produced in response to stress. We hypothesised STB-EV 5’-tRF release might change in preeclampsia.
Methods
We perfused placentas from eight women with early-onset preeclampsia and six controls, comparing small RNA expression in STB-EVs. We used membrane-affinity columns to isolate maternal plasma vesicles and investigate placental 5’-tRFs in-vivo . We quantified 5’-tRFs from circulating STB-EVs using a placental alkaline phosphatase immunoassay. 5’-tRFs and scrambled RNA controls were added to monocyte, macrophage and endothelial cells in culture to investigate transcriptional responses.
Results
5’-tRFs constitute the majority of small RNA in STB-EVs from both preeclampsia and normal pregnancies. >900 small RNA fragments are differentially expressed in preeclampsia STB-EVs. Preeclampsia-dysregulated 5’-tRFs are detectable in maternal plasma, where we identified a placentally-derived load. 5’-tRF-Glu-CTC, the most abundant preeclampsia-upregulated 5’-tRF in perfusion STB-EVs, is also increased in preeclampsia STB-EVs from maternal plasma. 5’-tRF-Glu-CTC induced inflammation in macrophages but not monocytes. The conditioned media from 5’’-tRF-Glu-CTC-activated macrophages reduced eNOS expression in endothelial cells.
Conclusions
Increased release of syncytiotrophoblast-derived vesicle-bound 5’-tRF-Glu-CTC contributes to preeclampsia pathophysiology.
