Daytime variation in SARS-CoV-2 infection and cytokine production

  1. Aïssatou Bailo Diallo
  2. Laetitia Gay
  3. Benjamin Coiffard
  4. Marc Leone
  5. Soraya Mezouar
  6. Jean-Louis Mege

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Abstract

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  1. Version published to 10.1016/j.micpath.2021.105067
  2. ScreenIT

    SciScore for 10.1101/2020.09.09.290718: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
    anti-SARS-CoV-2
    suggested: (Thermo Fisher Scientific Cat# 51-6490-82, RRID:AB_2884044)
    anti-rabbit
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Cells and virus: SARS-CoV-2 strain MI6 was cultured in Vero E6 cells (American type culture collection ATCC® CRL-1586™) in Minimum Essential Media (Life Technologies, Carlsbad, CA, USA) supplemented with 4% fetal bovine serum (FBS), as previously described (18).
    Vero E6
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistical analysis: Statistical analyses were performed with GraphPad Prism (7.0, La Jolla, CA) and R studio v3.4.0.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.09.09.290718v1 on bioRxiv