Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters
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SciScore for 10.1101/2020.06.16.151704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding This evaluation was performed by three experienced investigators blinded to the animal treatment. Power Analysis not detected. Sex as a biological variable Twelve female golden Syrian hamsters (eight weeks old) were purchased from Janvier’s breeding Centre (Le Genest, St Isle, France). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid Protein (1:200; rabbit polyclonal, NB100-56576, NOVUS, France); olfactory marker protein - OMP (1:500; goat polyclonal; 544-10001, WAKO, USA), … SciScore for 10.1101/2020.06.16.151704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding This evaluation was performed by three experienced investigators blinded to the animal treatment. Power Analysis not detected. Sex as a biological variable Twelve female golden Syrian hamsters (eight weeks old) were purchased from Janvier’s breeding Centre (Le Genest, St Isle, France). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid Protein (1:200; rabbit polyclonal, NB100-56576, NOVUS, France); olfactory marker protein - OMP (1:500; goat polyclonal; 544-10001, WAKO, USA), cytokeratin-18 - CK18 (1:50; mouse polyclonal; MAB3234 – RGE53, Sigma-Aldrich, France), ionized calcium-binding adapter molecule 1 - Iba1 (1: 1000; goat polyclonal; ab178846, Abcam, France); G alpha s/olf - Golf (1:500; rabbit polyclonal; Santa Cruz Biotechnology (sc383), Dallas, TX, USA). SARS Nucleocapsid Proteinsuggested: (LSBio (LifeSpan Cat# LS-C59557-500, RRID:AB_1510641)cytokeratin-18 - CK18suggested: Noneionized calcium-binding adapter molecule 1suggested: (Abcam Cat# ab178846, RRID:AB_2636859)Iba1suggested: (Abcam Cat# ab178846, RRID:AB_2636859)Fluorescence staining was performed using goat anti-rabbit Alexa-Fluor-488 (1:1000; Molecular Probes – A32731; Invitrogen, Cergy Pontoise, France), donkey anti-goat Alexa-Fluor-555 (1:1000; Molecular Probes A32816; Invitrogen, Cergy Pontoise, France) and donkey anti-mouse Alexa-Fluor 555 (1:500; Molecular Probes – A32773; Invitrogen, Cergy Pontoise, France) secondary antibodies. anti-rabbitsuggested: (Molecular Probes Cat# A-21429, RRID:AB_2535850)anti-goatsuggested: (Molecular Probes Cat# A-21432, RRID:AB_141788)anti-mousesuggested: (Molecular Probes Cat# A-21127, RRID:AB_141596)Experimental Models: Cell Lines Sentences Resources A volume of 400 μl was homogenized and microfiltered (0.5 μm) previous to inoculation on Vero cells CCL-81 (passage 32, from ATCC, USA) grown at 80% confluence in a BSL3 virology laboratory (Virology Unit, CHU de Caen, France). Verosuggested: NoneSoftware and Algorithms Sentences Resources Images were quantified using ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2012) to threshold specific staining. ImageJsuggested: (ImageJ, RRID:SCR_003070)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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